Product description

 

Ribonuclease R (RNase R) is a magnesium-dependent 3'→5' exoribonuclease that can digest essentially all linear RNAs, but does not digest lariat or circular RNA structures, or double-stranded RNA with 3' overhangs shorter than 7 nucleotides. As such, this enzyme is ideally suited to the study of lariat RNA produced by traditional splicing, as well as circRNAs which arise through back-splicing. By removing linear RNAs from cellular or RNA extracts, RNase R greatly facilitates the identification of circular species through RNA-sequencing. This enables researchers to probe the landscape of splicing events with greater depth.

This product is produced in accordance with GMP process requirements and provided in liquid form.

 

Specifications

 

Expression Host	Recombinant E. coli with RNase R gene
Storage Buffer	50 mM Tris-HCl, 100 mM NaCl, 0.1 mM EDTA, 1mM DTT, 0.1% TritonX-100，pH 7.5
Reaction Temperature	37℃
Unit Definition	One unit converts 1 μg of poly-r(A) into acid-soluble nucleotides in 10 minutes at 37 ℃.
Application	1.Identification of intronic lariat sequences 2.Identification of exonic circRNAs 3.Studying alternative splicing 4.Production of artificial circular RNAs

 

Storage

 

This product should be stored at -25~-15℃ for  two years.

 

Instructions

 

Experimental methods

1. The following reaction system was formulated in a sterile micro-centrifuge tube:

Components	Volume
10 × RNase R Reaction Buffer*	2 μL
RNA Sample	1 μg
RNase R (20 U/μL)	2-4 U
DEPC H2O	Up to 20 μL

^*According to the specific purpose of experiments, it is necessary to prepare the corresponding reaction buffer. You can consider buying 14616ES(10×RNase R Reaction Buffer GMP-grade) to use together.

2. Reaction condition：37℃ for 10 min to 30 min.
3. Inactivation condition: incubation at 70℃ for 10 min can inactivate the enzyme.

 

Notes

 

1. Please operate with lab coats and disposable gloves，for your safety.

 

Ver.EN20240407