Pyrophosphatase, Inorganic GMP-grade (1 U/μL) is an inorganic pyrophosphatase derived from recombinant expression in E. coli. It is an enzyme that can catalyze the conversion of one molecule of pyrophosphate into two molecules of phosphate ions. This is a high-energy reaction, so this reaction can be coupled to some thermodynamically unfavorable transformations in order to drive these transformations to completion. Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate. In molecular biology, it can be used to increase RNA yield in reverse transcription reaction.

This product is produced in accordance with GMP process requirements, and the product is provided in liquid form

 

Product Properties

Source	Recombinant E.coli with   Pyrophosphatase gene from yeast
Optimum   Temperature	25℃
Storage   Buffer	50 mM   Tris-HCl pH 7.9, 100 mM NaCl, 10 mM DTT, 1 mM EDTA, 0.1% （v/v）TritonX-100, 50%（v/v）glycerin
Unit   Definition	One unit (U) is the amount of enzyme needed   to catalyze the hydrolysis of PPI per minute to produce 1 μmol Pi.（The standard reaction is: 500 μL system contains 100 mM   Tris-HCl pH7.2, 2 mM MgCl2, 2 mM Ppi; reaction at 25℃ for 10 min）

 

Contents

Catalog No.	Name	Catalog No./Specification
		10620ES10 (10 U)	10620ES60 (100 U)	10620ES80 (1,000   U)	10620ES99 (40 KU)
10620	Pyrophosphatase, Inorganic   GMP-grade (1 U/μL)	10 μL	100 μL	1 mL	40 mL

 

Shipping and Storage

The Pyrophosphatase, Inorganic GMP-grade products shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.

 

Notes

1. This product is active in a variety of reaction buffers. Usually, this product can be directly connected in experiments such as IVT.

2.The optimal reaction temperature of this product is 25℃, it is active at 16-37℃, 65℃ can inactivate the enzyme for 10 minutes.

3. For your safety and health, please wear personal protective equipment (PPE), such as laboratory coats and disposable gloves, when operating with this. 

HB220701