Chicken DNA virus sensor DDX41 activates IFN-β signaling pathway dependent on STING
Yuqiang Cheng, Yunxia Liu, Yingying Wang, Qiaona Niu, Quanxin Gao, Qiang Fu, Jingjiao Ma, Hengan Wang, Yaxian Yan, Chan Ding, Jianhe Sun
Journal：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
IF：3.22
DOI：10.1016/j.dci.2017.07.001
PMID：28684273
Published：2017-07-03
research field：分子生物学免疫学病毒学
Abstract
The recognition of pathogenic DNA is important to the initiation of antiviral responses. Here, we report the identification of the first avian DEAD (Asp-Glu-Ala-Asp) box polypeptide 41 (DDX41), an important DNA sensor, in chicken cells. In our study, we confirmed that chDDX41 is not an interferon-inducible gene. Knockdown of chDDX41 expression by shRNA blocked the ability of DF-1 cells to mount an IFN-β response to DNA and associated viruses. ChDDX41 mRNAs could be upregulated by double-stranded DNA (dsDNA) analogue poly(dA:dT), but not by double-stranded RNA (dsRNA) analogue poly(I:C). In poly(dA:dT) stimulation assays, the immune molecules involved in the DDX41-mediated IFN-β pathway in human cells were universally upregulated in chicken cells. Via coimmunoprecipitation (Co-IP) experiments, we found that chDDX41 could strongly interact with chicken stimulator of IFN genes (chSTING). Therefore, our results suggest that chDDX41 is involved in the dsDNA- and dsDNA virus-mediated chDDX41-chSTING-IFN-β signaling pathway in chicken cells.
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