T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon (CD3E), CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

高纯度、高活性、低内毒素、高批间一致性

            SDS-PAGE

    2μg (R: reducing condition, N: non-reducing condition).

             SEC-HPLC

The purity of Biotinylated CD3E&CD3D Heterodimer, His,Avi Tag&Tag Free Protein, Human is more than 95% determined by SEC-HPLC.

           ELISA

Immobilized Biotinylated CD3E&CD3D Heterodimer, His,Avi Tag&Tag Free Protein, Human  at 1.0μg/mL (100μL/well) can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (UCHT1)) with EC50 of 1.37-2.03 ng/mL.

Immobilized Biotinylated CD3E&CD3D Heterodimer, His,Avi Tag&Tag Free Protein, Human (Cat. No. UA016084) at 1 μg/mL (100 μL/well) on Streptavidin at 1.0μg/mL (100μL/well) can bind NA/LE Mouse anti-human CD3 mAb  with EC50 of 2.43-5.41 ng/mL.

  SPR  Data

Biotinylated CD3E&CD3D Heterodimer, His, Avi Tag&Tag Free Protein, Human captured on Biotin CAP - Series S sensor Chip can bind NA/LE Mouse anti-human CD3 mAb with an affinity constant of 0.92nM as determined in a SPR assay.

-25 ~ -15℃保存，收到货之后有效期1年。复溶后， 无菌条件下，-85 ~ -65℃保存，3个月有效期。复溶后， 无菌条件下，2 ~ -8℃保存，7天有效期。