产品说明书
FAQ
COA
已发表文献
T-cell surface glycoprotein CD3 epsilon & CD3 delta chain, also known as CD3E & CD3D , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
产品性质
|
别名（Synonyms） |
CD3; CD3e; CD3E; CD3d; T3D; CD3D; CD3E&CD3D; CD3 delta&CD3 epsilon |
|
Uniprot No. |
P07766(CD3E)&P04234(CD3D) |
|
表达区间及表达系统（Source） |
Recombinant Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Asp23-Glu120 (CD3E)&Phe22-Ala105 (CD3D). |
|
分子量(Molecular Weight) |
The protein has a predicted MW of 17.9 kDa (CD3E) and 16.7 kDa (CD3D). Due to glycosylation, the protein migrates to 18-25 kDa based on Tris-Bis PAGE result. |
|
外观(Physical Appearance） |
Sterile Filtered White lyophilized (freeze-dried) powder. |
|
纯度（Purity） |
> 95% as determined by SDS-PAGE and HPLC |
|
活性（Activity） |
1.ELISA Data：Immobilized Human CD3E&CD3D at 2μg/ml (100μl/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 38ng/ml determined by ELISA. 2.ELISA Data：Immobilized Human CD3E&CD3D at 2ug/ml (100μl/Well). Dose response curve for Foralumab, hFc Tag with the EC50 of 18.5ng/ml determined by ELISA. |
|
内毒素（Endotoxin） |
< 1.0 EU per 1μg of the protein by the LAL method. |
|
制剂（Formulation） |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
|
复溶（Reconstitution） |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
运输与保存方法
冰袋运输。-20℃至-80℃保存，一年有效期。
复溶后，-20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。
建议第一次使用时分装冻存，避免反复冻融。
注意事项
1.避免反复冻融。
2.为了您的安全和健康，请穿实验服并戴一次性手套操作。
3.本产品仅作科研用途！
产品数据
Tris-Bis PAGE
Human CD3E&CD3D on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
SEC-HPLC
The purity of Human CD3E&CD3D is greater than 95% as determined by SEC-HPLC.
ELISA Data
Immobilized Human CD3E&CD3D at 2μg/ml (100μl/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 38ng/ml determined by ELISA.
ELISA Data
Immobilized Human CD3E&CD3D at 2ug/ml (100μl/Well).
Dose response curve for Foralumab, hFc Tag with the EC50 of 18.5ng/ml determined by ELISA.
HB231023
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