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Recombinant Human DLL3 (His Tag)
产品信息
|
产品名称 |
产品编号 |
规格 |
|
Recombinant Human DLL3 (His Tag) |
93757ES25 |
25 μg |
|
93757ES60 |
100 μg |
|
|
93757ES76 |
500 μg |
产品描述
Delta-like protein 3 (DLL3) is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Mature human DLL3 consists of a 466 amino acid (aa) extracellular domain (ECD) with one DSL domain and six EGF-like repeats, a 21 aa transmembrane segment, and a 105 aa cytoplasmic domain. Within the ECD, human DLL3 shares 86% aa sequence identity with mouse and rat DLL3. DLL3 is known as a divergent DSL ligand since it does not function in similar manner as the rest of DSL ligands. It does not activate Notch signaling through trans-activation, but autonomously attenuates signaling induced by other DSL ligands. A loss-of-function mutation of DLL3 is linked to axial skeletal defects in the spondylocostal dysplasia, which is linked to abnormal Notch signaling. DL-3 promotes proliferation and inhibits apoptosis of cancer cells. The proliferative effect mediated by DLL3 is thought to be due to increased Akt phosphorylation in cancer cells.
产品性质
|
别名（Synonyms） |
Delta3; DLL3; Pudgy; SCDO1; SCDO1delta3 |
|
Uniprot No. |
|
|
表达区间及表达系统（Source） |
Recombinant Human DLL3 is expressed from HEK293 Cells with His tag at the N-terminal. It contains Ala27-Arg490. |
|
分子量(Molecular Weight) |
The protein has a predicted MW of 50.4 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result. |
|
外观(Physical Appearance） |
Sterile Filtered White lyophilized (freeze-dried) powder. |
|
纯度（Purity） |
> 95% as determined by SDS-PAGE and HPLC |
|
活性（Activity） |
ELISA Data：Immobilized Human DLL3, His Tag at 0.1μg/mL (100μl/well). Dose response curve for Anti- DLL3 Antibody, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA. |
|
内毒素（Endotoxin） |
< 1.0 EU per 1μg of the protein by the LAL method. |
|
制剂（Formulation） |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
|
复溶（Reconstitution） |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
运输与保存方法
冰袋运输。-20℃至-80℃保存，一年有效期。
复溶后，-20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。
建议第一次使用时分装冻存，避免反复冻融。
注意事项
- 避免反复冻融。
- 为了您的安全和健康，请穿实验服并戴一次性手套操作。
- 本产品仅作科研用途！
产品数据
Tris-Bis PAGE
SEC-HPLC
ELISA Data
HB240419
