产品说明书
FAQ
COA
已发表文献
CD79B (also known as B29, Ig beta and B cell antigen receptor complex-associated protein beta-chain) is a 36-40 kDa member of the Ig-Superfamily. It is expressed on B cells, and forms a covalent heterodimer with CD79A. This complex interacts non-covalently with membrane Ig, forming the B cell antigen receptor. Within this complex, membrane Ig detects antigen while CD79AB initiates signaling. Mature human CD79B is a 201 amino acid (aa) type I transmembrane glycoprotein (aa 29229). It contains an extracellular region with one V-type Ig-like domain (aa 38-138) and an ITAM-containing cytoplasmic domain (aa 185-213). There is an alternative splice form that shows a deletion of aa 41-144 and appears after B cell activation. Human CD79A and B share only 26% aa identity. Over aa 29-159, human CD79B shares 54% aa identity with mouse CD79B.
产品性质
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别名（Synonyms） |
B29; CD79b molecule; CD79B; IGB; IGBAGM6; Ig-beta; Ig-β |
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Uniprot No. |
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表达区间及表达系统（Source） |
Recombinant Human CD79B Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Ala29-Asp159. |
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分子量(Molecular Weight) |
The protein has a predicted MW of 18.1 kDa. Due to glycosylation, the protein migrates to 33-42 kDa based on Tris-Bis PAGE result. |
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外观(Physical Appearance） |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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纯度（Purity） |
> 95% as determined by SDS-PAGE and HPLC |
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活性（Activity） |
ELISA Data：Immobilized Human CD79B, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-CD79b Ab., hFc Tag with the EC50 of 15.3ng/ml determined by ELISA. Batch to Batch Consistency: Immobilized Human CD79B, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-CD79B Ab., hFc Tag with the EC50 of 15.9/15.3/15.2ng/ml determined by ELISA. |
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内毒素（Endotoxin） |
< 1.0 EU per 1μg of the protein by the LAL method. |
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制剂（Formulation） |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
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复溶（Reconstitution） |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
运输与保存方法
冰袋运输。-20℃至-80℃保存，一年有效期。
复溶后，-20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。
建议第一次使用时分装冻存，避免反复冻融。
注意事项
1. 避免反复冻融。
2. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
3. 本产品仅作科研用途！
产品数据
Tris-Bis PAGE
HB220624
