产品说明书
FAQ
COA
已发表文献
CSF1R also known as M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region. The human M-CSF R ECD shares 60%, 64%, 72%, 75%, 75% and 76% aa identity with mouse, rat, bovine, canine, feline and equine M-CSF R, respectively. M-CSF R engagement is continuously required for macrophage survival and regulates lineage decisions and maturation of monocytes, macrophages, osteoclasts and DC. M-CSF R and integrin alpha v beta 3 share signaling pathways during osteoclastogenesis and deletion of either causes osteopetrosis. In the brain, microglia expressing increased M-CSF R are concentrated with Alzheimers a beta peptide, but their role in pathogenesis is unclear.
产品性质
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别名（Synonyms） |
CSF1R, CSF-1-R, CSFR, FMSFIM2, MCSFR, M-CSFR, C-FMS, FIM2, FMS, HDLS |
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Uniprot No. |
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表达区间及表达系统（Source） |
HEK293 Cells-derived Human M-CSF R / CSF1R / CD115 Protein, Ile20-Glu512 with hFc tag at the C-terminal |
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分子量(Molecular Weight) |
Approximately 81.3 kDa. Due to glycosylation, the protein migrates to 100-130 kDa based on Tris-Bis PAGE result. |
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外观(Physical Appearance） |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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纯度（Purity） |
> 95% as determined by SDS-PAGE and HPLC |
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活性（Activity） |
1.ELISA Data: Immobilized Human M-CSF at 0.5 μg/mL (100 μL/Well) on the plate. Dose response curve for Human M-CSF R, hFc Tag with the EC50 of 12.4 ng/mL determined by ELISA. 2.SPR Data: Human M-CSF, His Tag captured on CM5 Chip via Anti-his Antibody can bind Human M-CSF R, hFc Tag with an affinity constant of 0.24 nM as determined in SPR assay (Biacore T200). |
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内毒素（Endotoxin） |
< 1.0 EU per 1μg of the protein by the LAL method. |
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制剂（Formulation） |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
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复溶（Reconstitution） |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
运输与保存方法
冰袋运输。-20℃至-80℃保存，一年有效期。
复溶后，-20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。
建议第一次使用时分装冻存，避免反复冻融。
注意事项
1. 避免反复冻融。
2. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
3. 本产品仅作科研用途！
产品数据
HB220629
