产品说明书
FAQ
COA
已发表文献
Four distinct genes encoding closely related FGF receptors, FGF R1 - 4. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Only the alpha isoform has been identified for FGF R3 and FGF R4. Fibroblast growth factors (FGFs) comprise a family of at least eighteen structurally related proteins that are involved in a multitude of physiological and pathological cellular processes, including cell growth, differentiation, angiogenesis, wound healing and tumorgenesis.
产品性质
|
别名（Synonyms） |
FGF R2a, FGFR2 alpha, KGFR, CD332, BBDS, BEK, BFR-1, CEK3, CFD1, ECT1, JWS, K-SAM, KGFR, TK14, TK25, FLJ98662 |
|
Uniprot No. |
|
|
表达区间及表达系统（Source） |
Recombinant Human FGFR2 alpha (IIIb) Protein is expressed from HEK293 Cells with hFc tag at the C-terminal. It contains Arg22-Glu378. |
|
分子量(Molecular Weight) |
Approximately 66.4 kDa. Due to glycosylation, the protein migrates to 80-115 kDa based on Tris-Bis PAGE result. |
|
外观(Physical Appearance） |
Sterile Filtered White lyophilized (freeze-dried) powder. |
|
纯度（Purity） |
> 95% as determined by SDS-PAGE and HPLC |
|
活性（Activity） |
ELISA Data: Immobilized Human FGFR2 alpha IIIb, hFc tag at 0.5 μg/mL (100 μL/Well). Dose response curve for Biotinylated Anti-FGFR2 Ab., hFc Tag with the EC50 of 10.0 ng/mL determined by ELISA. |
|
内毒素（Endotoxin） |
< 1.0 EU per 1μg of the protein by the LAL method. |
|
制剂（Formulation） |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
|
复溶（Reconstitution） |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
运输与保存方法
冰袋运输。-20℃至-80℃保存，一年有效期。
复溶后，-20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。
建议第一次使用时分装冻存，避免反复冻融。
注意事项
1. 避免反复冻融。
2. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
3. 本产品仅作科研用途！
产品数据
Tris-Bis PAGE
HB220706
