T cell surface antigen CD2 is a type I glycoprotein belonging to the Ig superfamily and is expressed on T cells, NK cells, B cells and some antigen presenting cells. CD2 functions as an activator of T lymphocytes and thymocytes. Mature human CD2 consists of a 185 amino acid (aa) extracellular domain (ECD), a 26 aa transmembrane segment, and a 116 aa cytoplasmic tail. The extracellular domain is composed of two immunoglobulin-superfamily domains with highly-charged binding regions. Within the ECD, human CD2 shares 47% and 48% aa sequence identity with mouse and rat CD2, respectively. It interacts with both CD58 and CD59 directly to activate T cells and their adhesion pathways. The conformational flexibility of the CD2 molecule affects function through the conformational status of the adhesive ligands. Together with PSTPIP1, CD2 works to regulate T cell activation. These two proteins colocalize with a second CD2-binding protein to signal immunological synapse formation in T cells. CD2 is an adhesion molecule present on the cell surface of T cells, natural killer (NK) cells, and B cells; and its interaction with CD58 on antigen-presenting cells plays an important role in their immune reaction. CD2-CD58 interactions play a critical role in the anti-tumor immune response, and restoration of this signaling is an important strategy for anti-tumor therapy.

 

产品性质

别名（Synonyms）	T11; CD2; SRBC; LFA-2; LFA-3 receptor
Uniprot No.	P06729
表达区间及表达系统（Source）	Recombinant Human CD2/SRBC Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Lys25-Asp209.
分子量(Molecular Weight)	The protein has a predicted MW of 22.3 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
外观(Physical Appearance）	Sterile Filtered White lyophilized (freeze-dried) powder.
纯度（Purity）	> 95% as determined by SDS-PAGE and HPLC
活性（Activity）	SPR Data：Human CD58, hFc Tag captured on CM5 Chip via Protein A can bind Human CD2, His Tag with an affinity constant of 440 nM as determined in SPR assay (Biacore T200).
内毒素（Endotoxin）	< 1.0 EU per 1μg of the protein by the LAL method.
制剂（Formulation）	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
复溶（Reconstitution）	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

 

运输与保存方法

冰袋运输。-20℃至-80℃保存，一年有效期。

复溶后，-20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。

建议第一次使用时分装冻存，避免反复冻融。

 

注意事项

1. 避免反复冻融。

2. 为了您的安全和健康，请穿实验服并戴一次性手套操作。

3. 本产品仅作科研用途！

 

产品数据

Tris-Bis PAGE

 

 

HB220624