Hifair® Ⅲ One Step RT-qPCR Probe Kit是以RNA为模板进行定量PCR反应的试剂盒。在实验的过程中,逆转录和定量PCR在同一反应管中进行,简化了实验操作,降低了污染的风险。本试剂盒以RNA为模板,使用基因特异性引物,利用耐热Hifair® Ⅲ Reverse Transcriptase高效合成第一链cDNA,并搭配使用探针和UNICON® HotStart Taq DNA Polymerase,配合优化的缓冲体系能够特异灵敏的对RNA模板进行精确定量。
本试剂盒以预混的Mix形式提供,2×Hifair® Ⅲ P buffer包含优化的缓冲体系和dNTPs等,优化了有效抑制非特异性 PCR扩增的因子和提升PCR反应扩增效率的因子,适用于荧光标记探针的高特异性检测系统。此外,Hifair® UH Ⅲ Enzymes包含比例优化的Hifair® Ⅲ Reverse Transcriptase、RNase inhibitor及UNICON® HotStart Taq DNA Polymerase等。
产品组分
组分编号 | 组分名称 | 11145ES50 (50 T) | 11145ES70 (200 T) |
---|---|---|---|
11145-A | 2×Hifair® Ⅲ P buffer | 500 μL | 2×1 mL |
11145-B | Hifair® UH Ⅲ Enzymes | 50 μL | 200 μL |
11145-C | Hieff® 50×High Rox | 20 μL | 80 μL |
11145-D | Hieff® 50×Low Rox | 20 μL | 80 μL |
11145-E | RNase free ddH2O | 500 μL | 2×1 mL |
灵敏度高
冰袋运输。-20℃避光保存,有效期1年。本品避免反复冻融。建议分装保存。
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