产品说明书
FAQ
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已发表文献
产品描述
细胞裂解后的蛋白粗提物中含有大量的内源性蛋白酶，这些酶可以降解细胞提取物中的蛋白。因此为了防止蛋白纯化过程中目的蛋白不受蛋白酶的破坏，需要加入蛋白酶抑制剂对其进行活性抑制。
本品是多种常见蛋白酶抑制剂的混合物，包含AEBSF、Aprotinin、Bestatin、E-64、Leupeptin、Pepstatin A多种成分，广谱抑制来源于动植物、细菌、酵母等组织或细胞内的多种蛋白酶，如丝氨酸蛋白酶、半胱氨酸蛋白酶、氨肽酶、酸性蛋白酶等。另外，本品不含EDTA（一种金属蛋白酶抑制剂），特别适用于活性中心为金属离子依赖的蛋白。兼容于后续的固定化金属离子亲和层析（Immobilized metal-chelate affinity chromatography, IMAC）、2-D 凝胶电泳以及天然凝胶电泳等蛋白分析实验。
本品为溶于DMSO的溶液，以100×储存液的形式提供，可用于WB，Co-IP，Pull-down assay，IF，IHC，激酶研究等多种分析。
翌圣为您提供Western Blot实验整体解决方案，相关产品选购请参考：WB实验系列产品-选购指南
使用方法
使用前将本品取出回温至室温，样品分析前按照1:100（v/v）的比例加入到样品溶液中，混匀即可。
运输和保存方法
冰袋运输。-20℃保存，1年有效。
注意事项
1）本品具有广谱的蛋白酶抑制活性，但是不具有磷酸酶抑制剂活性。若进行磷酸化蛋白的研究，可使用我司的磷酸酶抑制剂混合物（100×）（Cat#20109 & Cat#20110）。
2）为了您的安全和健康，请穿实验服并戴一次性手套操作。
3）本产品仅作科研用途！
可能遇到的问题
|
问题 |
可能的原因 |
解决方案 |
|
抑制效果不佳 |
样品含有较高的蛋白酶 |
溶解该蛋白酶抑制剂片剂时提高其浓度 |
|
该片剂对特定的蛋白酶家族没有较好的抑制效果 |
该片剂中不包含该家族蛋白酶抑制剂组分 |
向蛋白体系中加入抑制该家族蛋白酶的抑制剂 |
|
起到抑制该家族的蛋白酶抑制剂组分浓度较低 |
溶解该蛋白酶抑制剂片剂时提高其浓度 |
Q：该产品作用范围是什么？
A：广谱抑制来源于动植物、细菌、酵母等组织或细胞内的丝氨酸蛋白酶、半胱氨酸蛋白酶、氨肽酶、酸性蛋白酶等。
Q：该产品的有何特点？
A：本品不含 EDTA，特别适用于活性中心为金属离子依赖的蛋白；兼容后续的固定化金属离子亲和层析凝胶电泳及天然凝胶电泳等蛋白分析实验。
Q：该产品与 20123 有何不同？
A：20124 成分与 20123 相同，以溶于DMSO 的溶液形式提供。二者均不含EDTA，EDTA 为金属蛋白酶抑制剂。区别在于 20123 为片剂，20124 为储液。
Q：怎么使用，和罗氏一样吗？
A：使用方法建议参考说明书。简便程度与罗氏相同，效果与罗氏无差别。已经有超过400 家实验室在使用。
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