DAPI，也称DAPI dihydrochloride，是一种常用的核酸染料，可以和双链DNA富含AT序列的小沟结合，产生比自身强20多倍的蓝色荧光。和EB(ethidium bromide)相比，DAPI对双链DNA的染色灵敏度要高很多倍。DAPI也可对RNA进行染色，染色机理是其可以选择性嵌入“AU序列”并发出荧光。相比DAPI-dsDNA(Ex/Em=358 nm/461 nm)，DAPI-RNA具有较长的最大发射波长(500 nm)，其荧光亮度仅有DAPI-dsDNA的20%。

尽管DAPI不能通过活细胞膜，但可以通过提高浓度使之进入活细胞。DAPI具有很高的光漂白承受水平，能用来检测酵母线粒体DNA，叶绿体DNA，病毒DNA，Microplasm DNA以及染色体DNA。

DAPI典型的蓝色荧光特性使其非常普遍的搭配其他绿色、黄色或红色荧光染料用于细胞生物学多色荧光标记技术，因此也常作为核酸和染色体的复染剂用于细胞凋亡检测、RNA原位杂交、直接或间接免疫检测等领域，其染色后可用荧光显微镜观察或流式细胞仪检测。

本产品为溶液，浓度为5 mg/mL。另外提供粉末形式的DAPI（Cat No. 40727ES10）供客户选购。

冰袋运输，5 mg/mL的DAPI储存液于-20ºC避光保存，1年有效。

Q:  DAPI是一种好的活细胞核标记探针吗？
A:  DAPI被认为是一种半渗透性/无渗透性的细胞核染料。对细胞核的染色性能好坏依赖于细胞系类型。某些细胞系可用DAPI染色，某些完全不可以，而某些标记结果不一致。替代的话，建议用Hoechst 33342或Hoechst 33258，具有同DAPI一样的波长和核酸结合模式，但是有非常好的细胞膜渗透性。

Q．DAPI和Hoechst染料相当类似，如何二选一？
A：DAPI是非常通用的蓝色荧光染料，用于细胞核复染。对固定和透化的细胞/组织，具有非常明亮的细胞核标记。然而，此染料被认为是半渗透至不渗透的染料，用于活细胞染色的结合不一致。Hoechst 33342是一种细胞膜渗透性的染料，具有同DAPI的结合机制和荧光特征。非常适合用于活细胞成像，效果就如同DAPI用于固定细胞染色。

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