Hifair®III 1st Strand cDNA Synthesis Kit (gDNA digester plus)是含有gDNA去除步骤的cDNA第一链合成试剂盒。该试剂盒基于Hifair®III Reverse Transcriptase而开发。该酶cDNA合成速度快,且热稳定性大幅度提高,可耐受高达60℃的反应温度,适合具有复杂二级结构的RNA模板的逆转录。同时,该酶增强了与模板的亲和力,适合少量模板以及低拷贝基因的逆转录。Hifair®III Reverse Transcriptase合成全长cDNA的能力也有了提升,可合成长达19.8 kb的cDNA。
该试剂盒包含gDNA digester Mix,可去除RNA模板中残留的基因组DNA污染,保证后续结果更加可靠。该试剂盒提供两种cDNA合成引物:Random Primers N6和Oligo (dT)18,用户可根据需要,选择Random Primers N6,Oligo (dT)18或Gene Specific Primers作为逆转录引物,合成的单链cDNA产物可直接用来进行后续PCR或者qPCR反应。
产品组分
组分编号 | 组分名称 | 11139ES10 | 11139ES60 |
---|---|---|---|
11139-A | RNase-free H2O | 1 mL | 2×1 mL |
11139-B | 5×gDNA Digester Mix | 30 μL | 300 μL |
11139-C | 10×Hifair® III Super Buffer | 20 μL | 200 μL |
11139-D | Hifair® III RT Enzyme Mix | 10 μL | 100 μL |
11139-E | Random Primers N6 (50 μM) | 10 μL | 100 μL |
11139-F | Oligo (dT)18 (50 μM) | 10 μL | 100 μL |
- 耐热性好:耐受60℃,适合具有复杂二级结构的RNA模板
- 引物灵活:可根据个人需求,搭配引物
- 兼容性高:适合不同GC含量、不同表达丰度基因反转录
- 合成能力强:最长可合成19.8kb的cDNA
-25~-15℃避光储存,有效期18个月。
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