产品说明书
FAQ
COA
已发表文献
产品描述
Hifair® Ⅱ 1st Strand cDNA Synthesis Kit是基于Hifair® Ⅱ Reverse Transcriptase开发的试剂盒。与Hieff® M-MLV (H-) Reverse Transcriptase相比，Hifair® Ⅱ Reverse Transcriptase热稳定性大幅度提高，可耐受高达50℃的反应温度，适合具有复杂二级结构的RNA模板的逆转录。同时，该酶增强了与模板的亲和力，适合少量模板以及低拷贝基因的逆转录。Hifair® Ⅱ Reverse Transcriptase合成全长cDNA的能力也有了提升，可扩增长达10 kb的cDNA。
试剂盒中包含由总RNA或mRNA合成高质量第一链cDNA所需的所有成分，并提供两种cDNA合成引物：Random Primers N6和oligo (dT)18，合成的单链cDNA产物可直接用来进行后续PCR或者qPCR反应。
产品组分
|
编号 |
组分 |
产品编号/规格 |
|
11119ES60 (100 T) |
||
|
11119-A |
RNase-free H2O |
2×1 mL |
|
11119-B |
5×Hifair® Ⅱ Buffer |
400 μL |
|
11119-C |
Hifair® Ⅱ Enzyme Mix |
200 μL |
|
11119-D |
Oligo (dT)18 (50 μM) |
100 μL |
|
11119-E |
Random Primers N6 (50 μM) |
100 μL |
【注】：1）5×Hifair® Ⅱ Buffer中包含dNTP。2）Hifair® Ⅱ Enzyme Mix中包含RNase inhibitor。
运输和保存方法
冰袋运输。-20℃保存。
注意事项
1）反应液的配制应在冰上操作完成，操作过程应避免RNase污染；
2) 为了您的安全和健康，请穿实验服并佩戴一次性手套操作。
3）本产品仅作科研用途！
第一链cDNA合成操作步骤
1. 逆转录反应体系配制（20 μL体系）
|
组分 |
使用量 |
|
RNase-free H2O |
To 20 μL |
|
Total RNA |
1 ng -5 μg* |
|
or mRNA |
1 ng-500 ng* |
|
5×Hifair® Ⅱ Buffer |
4 μL |
|
Random Primers N6 (50 μM) |
1 μL |
|
or Oligo (dT)18 (50 μM) |
or 1 μL |
|
or Gene Specific Primers (2 μM) |
or 1 μL |
|
Hifair® Ⅱ Enzyme Mix |
2 μL |
【可省略】：若后续实验为PCR，针对复杂模板，可将RNA、H2O、反转录引物在65℃孵育5 min 后，迅速冰上冷却，然后再加入Hifair® Ⅱ Enzyme Mix；若后续实验为qPCR，则可省略65℃孵育步骤，直接在体系中加入Hifair® Ⅱ Enzyme Mix。
【注】：* 若后续实验为qPCR，建议Total RNA 或 mRNA投入量不超过1 μg或100 ng，如果目的基因的表达丰度非常低，最多可投入5 μg Total RNA或500 ng mRNA。
2. 逆转录程序设置
|
温度 |
时间 |
|
25℃ |
5 min |
|
42℃ |
30 min |
|
85℃ |
5 min |
【注】：
1）荧光定量实验可以只使用Random Primers N6；也可以1:1与Oligo (dT)18混合使用，效果更好。
2）逆转录温度：推荐使用42℃。对于高GC含量模板或者复杂模板，可将逆转录温度提高到50℃。
3）逆转录产物可以-20℃短期保存，若需长期保存，建议分装后，于-80℃保存，避免反复冻融。
关于引物的选择
1） 如果模板为真核生物来源，建议选择Oligo (dT)18 ，与真核生物mRNA的3’ Poly A尾配对，可获得最高产量的全长cDNA。
2）原核生物RNA的反转录请选用Random Primers N6或者基因特异性引物。
3）Random Primers N6适用性较广，mRNA、rRNA、tRNA、small RNA和LncRNA等模板均可用Random Primers N6进行反转录。
4）使用Random primers N6，进行2 kb以下的cDNA合成时，Random primers N6的使用量为 1-2 μL；2 kb 以上的cDNA合成时，Random primers N6 的使用量为0.4-1 μL。
相关产品
|
产品名称 |
货号 |
规格 |
|
11119ES60 |
100 T |
|
|
11120ES60 |
100 T |
|
|
Hifair® II 1st Strand cDNA Synthesis Kit (gDNA digester plus) |
11121ES60 |
100 T |
|
Hifair®II 1st Strand cDNA Synthesis SuperMix for qPCR(gDNA digester plus) |
11123ES60 |
100 T |
|
Hifair® III 1st Strand cDNA Synthesis Kit (gDNA digester plus) |
11139ES60 |
100 T |
|
Hifair® Ⅲ 1st Strand cDNA Synthesis SuperMix for qPCR (gDNA digester plus) |
11141ES60 |
100 T |
|
11201ES08 |
5 mL |
|
|
11202ES08 |
5 mL |
|
|
11203ES08 |
5 mL |
|
|
Hieff UNICON® Power qPCR SYBR Green Master Mix ( 抗体法，No Rox) |
11195ES08 |
5 mL |
|
Hieff UNICON® Power qPCR SYBR Green Master Mix ( 抗体法，Low Rox) |
11196ES08 |
5 mL |
|
Hieff UNICON® Power qPCR SYBR Green Master Mix ( 抗体法，High Rox) |
11197ES08 |
5 mL |
|
11198ES08 |
5 mL |
|
|
11199ES08 |
5 mL |
|
|
11200ES08 |
5 mL |
|
|
11184ES08 |
5 mL |
HB210719
Q：A2800，promage 的货号， 用我们哪款反转录替换比较好点？
A：11119 是两种引物分开的，可以做 oligo（dT）和随机引物分开的，也可以混合的。如果客户只做定量的话，可以推 11137
Q：问一下 11110 这款反转录单酶，这款产品里头的组分有没有那款kit 或者 mix 也包含呢？
A：11120ES、11123ES、11119ES、11121ES
Q：Hifair Ⅱ 1st Strand cDNA Synthesis Kit 这个跟诺唯赞的 HiScript II Reverse Transcriptase 区别？
A：如果客户不要求逆转温度达到 55 度，是可以的，我们三代逆转录酶优于这个。
[1] Liu H, Cai Z, Wang F, et al. Colon-Targeted Adhesive Hydrogel Microsphere for Regulation of Gut Immunity and Flora. Adv Sci (Weinh). 2021;8(18):e2101619. doi:10.1002/advs.202101619(IF:16.806)
[2] Xu L, Xu S, Sun L, et al. Synergistic action of the gut microbiota in environmental RNA interference in a leaf beetle. Microbiome. 2021;9(1):98. Published 2021 May 4. doi:10.1186/s40168-021-01066-1(IF:14.652)
[3] Wang P, Yang P, Qian K, et al. Precise gene delivery systems with detachable albumin shell remodeling dysfunctional microglia by TREM2 for treatment of Alzheimer's disease. Biomaterials. 2022;281:121360. doi:10.1016/j.biomaterials.2021.121360(IF:12.479)
[4] Li H, Zhang Y, Liu M, et al. Targeting PDE4 as a promising therapeutic strategy in chronic ulcerative colitis through modulating mucosal homeostasis. Acta Pharm Sin B. 2022;12(1):228-245. doi:10.1016/j.apsb.2021.04.007(IF:11.614)
[5] Xiao Q, Chen Y, Liu CW, et al. MtNPF6.5 mediates chloride uptake and nitrate preference in Medicago roots. EMBO J. 2021;40(21):e106847. doi:10.15252/embj.2020106847(IF:11.598)
[6] Li Z, Song Y, He T, et al. M2 microglial small extracellular vesicles reduce glial scar formation via the miR-124/STAT3 pathway after ischemic stroke in mice. Theranostics. 2021;11(3):1232-1248. Published 2021 Jan 1. doi:10.7150/thno.48761(IF:11.556)
[7] Fang L, Li G, Sun Z, et al. CASB: a concanavalin A-based sample barcoding strategy for single-cell sequencing. Mol Syst Biol. 2021;17(4):e10060. doi:10.15252/msb.202010060(IF:11.429)
[8] Li C, Wang W, Sun Y, et al. Selective sorting and secretion of hY4 RNA fragments into extracellular vesicles mediated by methylated YBX1 to promote lung cancer progression. J Exp Clin Cancer Res. 2022;41(1):136. Published 2022 Apr 11. doi:10.1186/s13046-022-02346-w(IF:11.161)
[9] Tao L, Yi Y, Chen Y, et al. RIP1 kinase activity promotes steatohepatitis through mediating cell death and inflammation in macrophages. Cell Death Differ. 2021;28(4):1418-1433. doi:10.1038/s41418-020-00668-w(IF:10.717)
[10] Qin H, Qiu H, He ST, et al. Efficient disinfection of SARS-CoV-2-like coronavirus, pseudotyped SARS-CoV-2 and other coronaviruses using cold plasma induces spike protein damage. J Hazard Mater. 2022;430:128414. doi:10.1016/j.jhazmat.2022.128414(IF:10.588)
[11] Zhao Y, Cui J, Li Q, et al. A joint strategy comprising melatonin and 3-methyladenine to concurrently stimulate biomass and astaxanthin hyperaccumulation by Haematococcus pluvialis. Bioresour Technol. 2021;341:125784. doi:10.1016/j.biortech.2021.125784(IF:9.642)
[12] Qin Q, Shou J, Li M, et al. Stk24 protects against obesity-associated metabolic disorders by disrupting the NLRP3 inflammasome. Cell Rep. 2021;35(8):109161. doi:10.1016/j.celrep.2021.109161(IF:9.423)
[13] Yan P, Li Z, Xiong J, et al. LARP7 ameliorates cellular senescence and aging by allosterically enhancing SIRT1 deacetylase activity. Cell Rep. 2021;37(8):110038. doi:10.1016/j.celrep.2021.110038(IF:9.423)
[14] Liu Y, Hu H, Zanaroli G, Xu P, Tang H. A Pseudomonas sp. strain uniquely degrades PAHs and heterocyclic derivatives via lateral dioxygenation pathways. J Hazard Mater. 2021;403:123956. doi:10.1016/j.jhazmat.2020.123956(IF:9.038)
[15] Fang L, Wang W, Li G, et al. CIGAR-seq, a CRISPR/Cas-based method for unbiased screening of novel mRNA modification regulators. Mol Syst Biol. 2020;16(11):e10025. doi:10.15252/msb.202010025(IF:8.991)
[16] Bi G, Liang J, Zhao M, et al. miR-6077 promotes cisplatin/pemetrexed resistance in lung adenocarcinoma via CDKN1A/cell cycle arrest and KEAP1/ferroptosis pathways. Mol Ther Nucleic Acids. 2022;28:366-386. Published 2022 Mar 28. doi:10.1016/j.omtn.2022.03.020(IF:8.886)
[17] Hu B, Wang P, Zhang S, et al. HSP70 attenuates compression-induced apoptosis of nucleus pulposus cells by suppressing mitochondrial fission via upregulating the expression of SIRT3. Exp Mol Med. 2022;54(3):309-323. doi:10.1038/s12276-022-00745-9(IF:8.718)
[18] Qi HZ, Ye YL, Suo Y, et al. Wnt/β-catenin signaling mediates the abnormal osteogenic and adipogenic capabilities of bone marrow mesenchymal stem cells from chronic graft-versus-host disease patients. Cell Death Dis. 2021;12(4):308. Published 2021 Mar 23. doi:10.1038/s41419-021-03570-6(IF:8.469)
[19] Zou F, Qiu Y, Huang Y, et al. Effects of short-chain fatty acids in inhibiting HDAC and activating p38 MAPK are critical for promoting B10 cell generation and function. Cell Death Dis. 2021;12(6):582. Published 2021 Jun 7. doi:10.1038/s41419-021-03880-9(IF:8.469)
[20] Bai Z, Yang P, Yu F, et al. Combining adoptive NK cell infusion with a dopamine-releasing peptide reduces senescent cells in aged mice. Cell Death Dis. 2022;13(4):305. Published 2022 Apr 5. doi:10.1038/s41419-022-04562-w(IF:8.469)
[21] Li F, Li M, Zhu Q, et al. Imbalance of intestinal microbial homeostasis caused by acetamiprid is detrimental to resistance to pathogenic bacteria in Bombyx mori. Environ Pollut. 2021;289:117866. doi:10.1016/j.envpol.2021.117866(IF:8.071)
[22] Zhou W, Wang Y, Wang J, et al. β-Ionone causes endocrine disruption, hyperpigmentation and hypoactivity in zebrafish early life stages. Sci Total Environ. 2022;834:155433. doi:10.1016/j.scitotenv.2022.155433(IF:7.963)
[23] Luo J, Cheng Y, Guo L, Wang A, Lu M, Xu L. Variation of gut microbiota caused by an imbalance diet is detrimental to bugs' survival. Sci Total Environ. 2021;771:144880. doi:10.1016/j.scitotenv.2020.144880(IF:7.963)
[24] Xu M, Kong Y, Chen N, et al. Identification of Immune-Related Gene Signature and Prediction of CeRNA Network in Active Ulcerative Colitis. Front Immunol. 2022;13:855645. Published 2022 Mar 22. doi:10.3389/fimmu.2022.855645(IF:7.561)
[25] Lv W, Yu H, Han M, et al. Analysis of Tumor Glycosylation Characteristics and Implications for Immune Checkpoint Inhibitor's Efficacy for Breast Cancer. Front Immunol. 2022;13:830158. Published 2022 Apr 4. doi:10.3389/fimmu.2022.830158(IF:7.561)
[26] Zhai X, Kong WG, Cheng GF, et al. Molecular Characterization and Expression Analysis of Intercellular Adhesion Molecule-1 (ICAM-1) Genes in Rainbow Trout (Oncorhynchus mykiss) in Response to Viral, Bacterial and Parasitic Challenge. Front Immunol. 2021;12:704224. Published 2021 Aug 20. doi:10.3389/fimmu.2021.704224(IF:7.561)
[27] Xu M, Kong Y, Chen N, et al. Identification of Immune-Related Gene Signature and Prediction of CeRNA Network in Active Ulcerative Colitis. Front Immunol. 2022;13:855645. Published 2022 Mar 22. doi:10.3389/fimmu.2022.855645(IF:7.561)
[28] Zhao Y, Wang HP, Yu C, et al. Integration of physiological and metabolomic profiles to elucidate the regulatory mechanisms underlying the stimulatory effect of melatonin on astaxanthin and lipids coproduction in Haematococcus pluvialis under inductive stress conditions. Bioresour Technol. 2021;319:124150. doi:10.1016/j.biortech.2020.124150(IF:7.539)
[29] Li X, Zhang X, Zhao Y, Yu X. Cross-talk between gama-aminobutyric acid and calcium ion regulates lipid biosynthesis in Monoraphidium sp. QLY-1 in response to combined treatment of fulvic acid and salinity stress. Bioresour Technol. 2020;315:123833. doi:10.1016/j.biortech.2020.123833(IF:7.539)
[30] Chen Y, Su L, Huang C, et al. Galactosyltransferase B4GALT1 confers chemoresistance in pancreatic ductal adenocarcinomas by upregulating N-linked glycosylation of CDK11p110. Cancer Lett. 2021;500:228-243. doi:10.1016/j.canlet.2020.12.006(IF:7.360)
[31] Wang K, Chen Y, Gao S, et al. Norlichexanthone purified from plant endophyte prevents postmenopausal osteoporosis by targeting ER α to inhibit RANKL signaling. Acta Pharm Sin B. 2021;11(2):442-455. doi:10.1016/j.apsb.2020.09.012(IF:7.097)
[32] Luo Y, Li C, He T, et al. A highly branched α-d-glucan facilitates antitumor immunity by reducing cancer cell CXCL5 expression. Int J Biol Macromol. 2022;209(Pt A):166-179. doi:10.1016/j.ijbiomac.2022.03.217(IF:6.953)
[33] Zou H, Guan M, Li Y, Luo F, Wang W, Qin Y. Targeted gene correction and functional recovery in achondroplasia patient-derived iPSCs. Stem Cell Res Ther. 2021;12(1):485. Published 2021 Aug 28. doi:10.1186/s13287-021-02555-8(IF:6.832)
[34] Zhao Y, Wu J, Li D, et al. Human ESC-derived immunity- and matrix- regulatory cells ameliorated white matter damage and vascular cognitive impairment in rats subjected to chronic cerebral hypoperfusion. Cell Prolif. 2022;55(5):e13223. doi:10.1111/cpr.13223(IF:6.831)
[35] Liu J, Hou Z, Wu J, et al. Infusion of hESC derived Immunity-and-matrix regulatory cells improves cognitive ability in early-stage AD mice. Cell Prolif. 2021;54(8):e13085. doi:10.1111/cpr.13085(IF:6.831)
[36] Tang L, Liu M, Hu C, et al. Binary exposure to hypoxia and perfluorobutane sulfonate disturbs sensory perception and chromatin topography in marine medaka embryos. Environ Pollut. 2020;266(Pt 3):115284. doi:10.1016/j.envpol.2020.115284(IF:6.793)
[37] Liu M, Song S, Hu C, et al. Dietary administration of probiotic Lactobacillus rhamnosus modulates the neurological toxicities of perfluorobutanesulfonate in zebrafish. Environ Pollut. 2020;265(Pt B):114832. doi:10.1016/j.envpol.2020.114832(IF:6.793)
[38] Lou MD, Li J, Cheng Y, et al. Glucagon up-regulates hepatic mitochondrial pyruvate carrier 1 through cAMP-responsive element-binding protein; inhibition of hepatic gluconeogenesis by ginsenoside Rb1. Br J Pharmacol. 2019;176(16):2962-2976. doi:10.1111/bph.14758(IF:6.583)
[39] Han S, Cao D, Sha J, Zhu X, Chen D. LncRNA ZFPM2-AS1 promotes lung adenocarcinoma progression by interacting with UPF1 to destabilize ZFPM2. Mol Oncol. 2020;14(5):1074-1088. doi:10.1002/1878-0261.12631(IF:6.574)
[40] Zeng Y, Zhu G, Zhu M, et al. Edaravone Attenuated Particulate Matter-Induced Lung Inflammation by Inhibiting ROS-NF-κB Signaling Pathway. Oxid Med Cell Longev. 2022;2022:6908884. Published 2022 Apr 23. doi:10.1155/2022/6908884(IF:6.543)
[41] Jia F, Wu Q, Wang Z, et al. BOP1 Knockdown Attenuates Neointimal Hyperplasia by Activating p53 and Inhibiting Nascent Protein Synthesis. Oxid Med Cell Longev. 2021;2021:5986260. Published 2021 Jan 16. doi:10.1155/2021/5986260(IF:6.543)
[42] Zhang HH, Zhou XJ, Zhong YS, et al. Naringin suppressed airway inflammation and ameliorated pulmonary endothelial hyperpermeability by upregulating Aquaporin1 in lipopolysaccharide/cigarette smoke-induced mice. Biomed Pharmacother. 2022;150:113035. doi:10.1016/j.biopha.2022.113035(IF:6.530)
[43] Xu H, Zhao Y, Gao X, Wang F, Gu Y. An innovative fluorescent probe targeting IGF1R for breast cancer diagnosis. Eur J Med Chem. 2021;219:113440. doi:10.1016/j.ejmech.2021.113440(IF:6.514)
[44] Zeng Z, Lei S, He Z, Chen T, Jiang J. YEATS2 is a target of HIF1α and promotes pancreatic cancer cell proliferation and migration. J Cell Physiol. 2021;236(3):2087-2098. doi:10.1002/jcp.29995(IF:6.384)
[45] Li S, Hao M, Li B, et al. CACNA1H downregulation induces skeletal muscle atrophy involving endoplasmic reticulum stress activation and autophagy flux blockade. Cell Death Dis. 2020;11(4):279. Published 2020 Apr 24. doi:10.1038/s41419-020-2484-2(IF:6.304)
[46] Liu M, Tang L, Hu C, Sun B, Huang Z, Chen L. Interaction between probiotic additive and perfluorobutanesulfonate pollutant on offspring growth and health after parental exposure using zebrafish. Ecotoxicol Environ Saf. 2021;214:112107. doi:10.1016/j.ecoenv.2021.112107(IF:6.291)
[47] Zhou WD, Shao L, Dong L, et al. Circulating MicroRNAs as Quantitative Biomarkers for Diagnosis and Prognosis of Uveal Melanoma. Front Oncol. 2022;12:854253. Published 2022 Mar 31. doi:10.3389/fonc.2022.854253(IF:6.244)
[48] Lv W, Wang Y, Zhao C, et al. Identification and Validation of m6A-Related lncRNA Signature as Potential Predictive Biomarkers in Breast Cancer. Front Oncol. 2021;11:745719. Published 2021 Oct 15. doi:10.3389/fonc.2021.745719(IF:6.244)
[49] Lv W, Zhao C, Tan Y, et al. Identification of an Aging-Related Gene Signature in Predicting Prognosis and Indicating Tumor Immune Microenvironment in Breast Cancer. Front Oncol. 2021;11:796555. Published 2021 Dec 16. doi:10.3389/fonc.2021.796555(IF:6.244)
[50] Yu Q, Zhang W, Zhou X, Shen W, Xing C, Yang X. Regulation of lnc-TLCD2-1 on Radiation Sensitivity of Colorectal Cancer and Comprehensive Analysis of Its Mechanism. Front Oncol. 2021;11:714159. Published 2021 Jul 15. doi:10.3389/fonc.2021.714159(IF:6.244)
[51] Huang X, Zhao L, Jin Y, et al. Up-Regulated MISP Is Associated With Poor Prognosis and Immune Infiltration in Pancreatic Ductal Adenocarcinoma. Front Oncol. 2022;12:827051. Published 2022 Mar 30. doi:10.3389/fonc.2022.827051(IF:6.244)
[52] Cui Y, Liu Y, Mu L, Li Y, Wu G. Transcriptional Expressions of ALDH1A1/B1 as Independent Indicators for the Survival of Thyroid Cancer Patients. Front Oncol. 2022;12:821958. Published 2022 Feb 23. doi:10.3389/fonc.2022.821958(IF:6.244)
[53] Duan AQ, Tao JP, Jia LL, et al. AgNAC1, a celery transcription factor, related to regulation on lignin biosynthesis and salt tolerance. Genomics. 2020;112(6):5254-5264. doi:10.1016/j.ygeno.2020.09.049(IF:6.205)
[54] Li A, Liu Q, Li Q, Liu B, Yang Y, Zhang N. Berberine Reduces Pyruvate-driven Hepatic Glucose Production by Limiting Mitochondrial Import of Pyruvate through Mitochondrial Pyruvate Carrier 1. EBioMedicine. 2018;34:243-255. doi:10.1016/j.ebiom.2018.07.039(IF:6.183)
[55] Gao C, Wang SW, Lu JC, et al. KSR2-14-3-3ζ complex serves as a biomarker and potential therapeutic target in sorafenib-resistant hepatocellular carcinoma. Biomark Res. 2022;10(1):25. Published 2022 Apr 25. doi:10.1186/s40364-022-00361-9(IF:6.148)
[56] Ben XY, Wang YR, Zheng HH, et al. Construction of Exosomes that Overexpress CD47 and Evaluation of Their Immune Escape. Front Bioeng Biotechnol. 2022;10:936951. Published 2022 Jun 30. doi:10.3389/fbioe.2022.936951(IF:6.064)
[57] Pan J, Qu M, Li Y, et al. MicroRNA-126-3p/-5p Overexpression Attenuates Blood-Brain Barrier Disruption in a Mouse Model of Middle Cerebral Artery Occlusion. Stroke. 2020;51(2):619-627. doi:10.1161/STROKEAHA.119.027531(IF:6.058)
[58] Jia H, Zhang T, Liu N, et al. 4-Phenylbutyric acid alleviates 3-acetyldeoxynivalenol-induced immune cells response by inhibiting endoplasmic reticulum stress in mouse spleen. Food Chem Toxicol. 2022;164:113002. doi:10.1016/j.fct.2022.113002(IF:6.025)
[59] Xu XY, He XT, Wang J, et al. Role of the P2X7 receptor in inflammation-mediated changes in the osteogenesis of periodontal ligament stem cells. Cell Death Dis. 2019;10(1):20. Published 2019 Jan 8. doi:10.1038/s41419-018-1253-y(IF:5.959)
[60] He G, Yang P, Cao Y, et al. Cytokinin Type-B Response Regulators Promote Bulbil Initiation in Lilium lancifolium. Int J Mol Sci. 2021;22(7):3320. Published 2021 Mar 24. doi:10.3390/ijms22073320(IF:5.924)
[61] Li C, Qin F, Wang W, et al. hnRNPA2B1-Mediated Extracellular Vesicles Sorting of miR-122-5p Potentially Promotes Lung Cancer Progression. Int J Mol Sci. 2021;22(23):12866. Published 2021 Nov 28. doi:10.3390/ijms222312866(IF:5.924)
[62] Yuan L, Zhang L, Wei X, et al. Quantitative Trait Locus Mapping of Salt Tolerance in Wild Rice Oryza longistaminata. Int J Mol Sci. 2022;23(4):2379. Published 2022 Feb 21. doi:10.3390/ijms23042379(IF:5.924)
[63] Zou G, Ren J, Wu D, et al. Characterization and Heterologous Expression of UDP-Glucose 4-Epimerase From a Hericium erinaceus Mutant with High Polysaccharide Production. Front Bioeng Biotechnol. 2021;9:796278. Published 2021 Nov 25. doi:10.3389/fbioe.2021.796278(IF:5.890)
[64] Zhang X, Liu Y, Luo C, et al. Crosslinker-free silk/decellularized extracellular matrix porous bioink for 3D bioprinting-based cartilage tissue engineering. Mater Sci Eng C Mater Biol Appl. 2021;118:111388. doi:10.1016/j.msec.2020.111388(IF:5.880)
[65] Fan CL, Liu TB. The Vacuolar Morphogenesis Protein Vam6-Like Protein Vlp1 Is Required for Pathogenicity of Cryptococcus neoformans. J Fungi (Basel). 2021;7(6):418. Published 2021 May 27. doi:10.3390/jof7060418(IF:5.816)
[66] Duan Q, Liu T, Huang C, et al. The Chinese Herbal Prescription JieZe-1 Inhibits Membrane Fusion and the Toll-like Receptor Signaling Pathway in a Genital Herpes Mouse Model. Front Pharmacol. 2021;12:707695. Published 2021 Sep 24. doi:10.3389/fphar.2021.707695(IF:5.811)
[67] Lu R, Yu X, Liang S, et al. Physalin A Inhibits MAPK and NF-κB Signal Transduction Through Integrin αVβ3 and Exerts Chondroprotective Effect. Front Pharmacol. 2021;12:761922. Published 2021 Dec 1. doi:10.3389/fphar.2021.761922(IF:5.811)
[68] Hu C, Tang L, Liu M, Lam PKS, Lam JCW, Chen L. Probiotic modulation of perfluorobutanesulfonate toxicity in zebrafish: Disturbances in retinoid metabolism and visual physiology. Chemosphere. 2020;258:127409. doi:10.1016/j.chemosphere.2020.127409(IF:5.778)
[69] Yang X, Wang Z, Kai J, et al. Curcumol attenuates liver sinusoidal endothelial cell angiogenesis via regulating Glis-PROX1-HIF-1α in liver fibrosis. Cell Prolif. 2020;53(3):e12762. doi:10.1111/cpr.12762(IF:5.753)
[70] Li T, Liu X, Xu B, et al. SKA1 regulates actin cytoskeleton remodelling via activating Cdc42 and influences the migration of pancreatic ductal adenocarcinoma cells. Cell Prolif. 2020;53(4):e12799. doi:10.1111/cpr.12799(IF:5.753)
[71] Sun Y, Tang L, Liu Y, et al. Activation of aryl hydrocarbon receptor by dioxin directly shifts gut microbiota in zebrafish. Environ Pollut. 2019;255(Pt 3):113357. doi:10.1016/j.envpol.2019.113357(IF:5.714)
[72] Sun K, Luo J, Jing X, et al. Astaxanthin protects against osteoarthritis via Nrf2: a guardian of cartilage homeostasis. Aging (Albany NY). 2019;11(22):10513-10531. doi:10.18632/aging.102474(IF:5.682)
[73] Li X, Zhao C, Zhang T, et al. Exogenous Aspergillus aculeatus Enhances Drought and Heat Tolerance of Perennial Ryegrass. Front Microbiol. 2021;12:593722. Published 2021 Feb 19. doi:10.3389/fmicb.2021.593722(IF:5.640)
[74] Liu M, Sun Y, Tang L, et al. Fingerprinting fecal DNA and mRNA as a non-invasive strategy to assess the impact of polychlorinated biphenyl 126 exposure on zebrafish. J Environ Sci (China). 2021;106:15-25. doi:10.1016/j.jes.2021.01.016(IF:5.565)
[75] Qiu X, Wang W, Zhang L, Guo L, Xu P, Tang H. A thermophile Hydrogenibacillus sp. strain efficiently degrades environmental pollutants polycyclic aromatic hydrocarbons. Environ Microbiol. 2022;24(1):436-450. doi:10.1111/1462-2920.15869(IF:5.491)
[76] Yang JJ , Wang YH , Yin J , Leng H , Shen SD . Polysaccharides from Ulva prolifera O.F. Müller inhibit cell proliferation via activating MAPK signaling in A549 and H1650 cells. Food Funct. 2021;12(15):6915-6924. doi:10.1039/d1fo00294e(IF:5.396)
[77] Qu Y, Ji H, Song W, et al. The anti-fatigue effect of the Auxis thazard oligopeptide via modulation of the AMPK/PGC-1α pathway in mice. Food Funct. 2022;13(3):1641-1650. Published 2022 Feb 7. doi:10.1039/d1fo03320d(IF:5.396)
[78] Han LT, Wu YJ, Liu TB. The F-Box Protein Fbp1 Regulates Virulence of Cryptococcus neoformans Through the Putative Zinc-Binding Protein Zbp1. Front Cell Infect Microbiol. 2021;11:794661. Published 2021 Dec 27. doi:10.3389/fcimb.2021.794661(IF:5.293)
[79] Liu T, Yang R, Zhou J, et al. Interactions Between Streptococcus gordonii and Fusobacterium nucleatum Altered Bacterial Transcriptional Profiling and Attenuated the Immune Responses of Macrophages. Front Cell Infect Microbiol. 2022;11:783323. Published 2022 Jan 7. doi:10.3389/fcimb.2021.783323(IF:5.293)
[80] Zhang Y, Wu S, Liu Y, et al. Acute Cold Water-Immersion Restraint Stress Induces Intestinal Injury and Reduces the Diversity of Gut Microbiota in Mice. Front Cell Infect Microbiol. 2021;11:706849. Published 2021 Oct 14. doi:10.3389/fcimb.2021.706849(IF:5.293)
[81] Gan Z, Yuan X, Shan N, et al. AcERF1B and AcERF073 Positively Regulate Indole-3-acetic Acid Degradation by Activating AcGH3.1 Transcription during Postharvest Kiwifruit Ripening. J Agric Food Chem. 2021;69(46):13859-13870. doi:10.1021/acs.jafc.1c03954(IF:5.279)
[82] Xu S, Mo C, Lin J, et al. Loss of ID3 drives papillary thyroid cancer metastasis by targeting E47-mediated epithelial to mesenchymal transition. Cell Death Discov. 2021;7(1):226. Published 2021 Aug 30. doi:10.1038/s41420-021-00614-w(IF:5.241)
[83] Jiang C, He J, Xu S, Wang Q, Cheng J. NR4A1 promotes LEF1 expression in the pathogenesis of papillary thyroid cancer. Cell Death Discov. 2022;8(1):46. Published 2022 Feb 2. doi:10.1038/s41420-022-00843-7(IF:5.241)
[84] Zhang Y, Xu X, Ma J, et al. Loss of CD226 protects apolipoprotein E-deficient mice from diet-induced atherosclerosis. Biochim Biophys Acta Mol Basis Dis. 2022;1868(9):166452. doi:10.1016/j.bbadis.2022.166452(IF:5.187)
[85] Shi X, Huang X, Chen R, et al. The transcribed ultraconserved element uc.51 promotes the proliferation and metastasis of breast cancer by stabilizing NONO. Clin Exp Metastasis. 2021;38(6):551-571. doi:10.1007/s10585-021-10128-5(IF:5.150)
[86] Xu HY, Dong F, Zhai X, et al. Mediation of Mucosal Immunoglobulins in Buccal Cavity of Teleost in Antibacterial Immunity. Front Immunol. 2020;11:562795. Published 2020 Sep 23. doi:10.3389/fimmu.2020.562795(IF:5.085)
[87] Dong F, Yin GM, Meng KF, et al. IgT Plays a Predominant Role in the Antibacterial Immunity of Rainbow Trout Olfactory Organs. Front Immunol. 2020;11:583740. Published 2020 Nov 16. doi:10.3389/fimmu.2020.583740(IF:5.085)
[88] Lv X, Chen J, He J, et al. Gasdermin D-mediated pyroptosis suppresses liver regeneration after 70% partial hepatectomy [published online ahead of print, 2022 May 4]. Hepatol Commun. 2022;10.1002/hep4.1973. doi:10.1002/hep4.1973(IF:5.073)
[89] Zhang R, Wang YH, Shi X, Ji J, Zhan FQ, Leng H. Sortilin regulates keratinocyte proliferation and apoptosis through the PI3K-AKT signaling pathway. Life Sci. 2021;278:119630. doi:10.1016/j.lfs.2021.119630(IF:5.037)
[90] Wang X, Yao Z, Fang L. miR-22-3p/PGC1β Suppresses Breast Cancer Cell Tumorigenesis via PPARγ. PPAR Res. 2021;2021:6661828. Published 2021 Mar 12. doi:10.1155/2021/6661828(IF:4.964)
[91] Xu H, Tang Y, Zhao Y, et al. SPECT Imaging of Hepatocellular Carcinoma Detection by the GPC3 Receptor. Mol Pharm. 2021;18(5):2082-2090. doi:10.1021/acs.molpharmaceut.1c00060(IF:4.939)
[92] Cao Q, Luo J, Xiong Y, Liu Z, Ye Q. 25-Hydroxycholesterol mitigates hepatic ischemia reperfusion injury via mediating mitophagy. Int Immunopharmacol. 2021;96:107643. doi:10.1016/j.intimp.2021.107643(IF:4.932)
[93] Zhu Z, Hu R, Li J, et al. Alpinetin exerts anti-inflammatory, anti-oxidative and anti-angiogenic effects through activating the Nrf2 pathway and inhibiting NLRP3 pathway in carbon tetrachloride-induced liver fibrosis. Int Immunopharmacol. 2021;96:107660. doi:10.1016/j.intimp.2021.107660(IF:4.932)
[94] Sun L, Li X, Xu M, Yang F, Wang W, Niu X. In vitro immunomodulation of magnesium on monocytic cell toward anti-inflammatory macrophages. Regen Biomater. 2020;7(4):391-401. doi:10.1093/rb/rbaa010(IF:4.882)
[95] Liu W, Lin H, Mao Z, et al. Verapamil extends lifespan in Caenorhabditis elegans by inhibiting calcineurin activity and promoting autophagy. Aging (Albany NY). 2020;12(6):5300-5317. doi:10.18632/aging.102951(IF:4.831)
[96] Zhou X , Weng W , Chen B , et al. Mesoporous silica nanoparticles/gelatin porous composite scaffolds with localized and sustained release of vancomycin for treatment of infected bone defects. J Mater Chem B. 2018;6(5):740-752. doi:10.1039/c7tb01246b(IF:4.776)
[97] Gan Z, Yuan X, Shan N, et al. AcWRKY40 mediates ethylene biosynthesis during postharvest ripening in kiwifruit. Plant Sci. 2021;309:110948. doi:10.1016/j.plantsci.2021.110948(IF:4.729)
[98] Dong S, Ding LG, Cao JF, et al. Viral-Infected Change of the Digestive Tract Microbiota Associated With Mucosal Immunity in Teleost Fish. Front Immunol. 2019;10:2878. Published 2019 Dec 18. doi:10.3389/fimmu.2019.02878(IF:4.716)
[99] Tan XH, Zhang KK, Xu JT, et al. Luteolin alleviates methamphetamine-induced neurotoxicity by suppressing PI3K/Akt pathway-modulated apoptosis and autophagy in rats. Food Chem Toxicol. 2020;137:111179. doi:10.1016/j.fct.2020.111179(IF:4.679)
[100] Jiang Q, Zhang J, Li F, et al. POLR2A Promotes the Proliferation of Gastric Cancer Cells by Advancing the Overall Cell Cycle Progression. Front Genet. 2021;12:688575. Published 2021 Nov 25. doi:10.3389/fgene.2021.688575(IF:4.599)
[101] Wang X, Sun S, Cao X, Gao J. Quantitative Phosphoproteomic Analysis Reveals the Regulatory Networks of Elovl6 on Lipid and Glucose Metabolism in Zebrafish. Int J Mol Sci. 2020;21(8):2860. Published 2020 Apr 19. doi:10.3390/ijms21082860(IF:4.556)
[102] Zhou X, Zhang Q, Chen L, et al. Versatile Nanocarrier Based on Functionalized Mesoporous Silica Nanoparticles to Codeliver Osteogenic Gene and Drug for Enhanced Osteodifferentiation. ACS Biomater Sci Eng. 2019;5(2):710-723. doi:10.1021/acsbiomaterials.8b01110(IF:4.511)
[103] Wang T, Cheng C, Peng L, et al. Combination of arsenic trioxide and Dasatinib: a new strategy to treat Philadelphia chromosome-positive acute lymphoblastic leukaemia. J Cell Mol Med. 2018;22(3):1614-1626. doi:10.1111/jcmm.13436(IF:4.499)
[104] Nie W, Gao Y, McCoul DJ, et al. Rapid mineralization of hierarchical poly(l-lactic acid)/poly(ε-caprolactone) nanofibrous scaffolds by electrodeposition for bone regeneration. Int J Nanomedicine. 2019;14:3929-3941. Published 2019 May 27. doi:10.2147/IJN.S205194(IF:4.471)
[105] Zheng J, Wang JJ, Ma HM, Shen MQ, Qian ZM, Bao YX. Norcantharidin down-regulates iron contents in the liver and spleen of lipopolysaccharide-treated mice. Redox Rep. 2022;27(1):119-127. doi:10.1080/13510002.2022.2088011(IF:4.412)
[106] Zhang Y, Liu D, Xue F, et al. Anti-Malignant Ascites Effect of Total Diterpenoids from Euphorbiae Ebracteolatae Radix Is Attributable to Alterations of Aquaporins via Inhibiting PKC Activity in the Kidney. Molecules. 2021;26(4):942. Published 2021 Feb 10. doi:10.3390/molecules26040942(IF:4.412)
[107] Deng XX, Jiao YN, Hao HF, Xue D, Bai CC, Han SY. Taraxacum mongolicum extract inhibited malignant phenotype of triple-negative breast cancer cells in tumor-associated macrophages microenvironment through suppressing IL-10 / STAT3 / PD-L1 signaling pathways. J Ethnopharmacol. 2021;274:113978. doi:10.1016/j.jep.2021.113978(IF:4.360)
[108] Li L, Zhang Z, Huang Y. Integrative transcriptome analysis and discovery of signaling pathways involved in the protective effects of curcumin against oxidative stress in tilapia hepatocytes. Aquat Toxicol. 2020;224:105516. doi:10.1016/j.aquatox.2020.105516(IF:4.346)
[109] Gui W, Zhu WF, Zhu Y, et al. LncRNAH19 improves insulin resistance in skeletal muscle by regulating heterogeneous nuclear ribonucleoprotein A1. Cell Commun Signal. 2020;18(1):173. Published 2020 Oct 28. doi:10.1186/s12964-020-00654-2(IF:4.344)
[110] He Y, Qiu R, Wu B, et al. Transthyretin contributes to insulin resistance and diminishes exercise-induced insulin sensitivity in obese mice by inhibiting AMPK activity in skeletal muscle. Am J Physiol Endocrinol Metab. 2021;320(4):E808-E821. doi:10.1152/ajpendo.00495.2020(IF:4.310)
[111] Wang J, Yin R, Zhang X, et al. Transcriptomic analysis reveals ligninolytic enzymes of white-rot fungus Phanerochaete sordida YK-624 participating in bisphenol F biodegradation under ligninolytic conditions. Environ Sci Pollut Res Int. 2021;28(44):62390-62397. doi:10.1007/s11356-021-15012-z(IF:4.223)
[112] Gao L, Yang X, Liang B, et al. Autophagy-induced p62 accumulation is required for curcumol to regulate KLF5-mediated angiogenesis in liver sinusoidal endothelial cells. Toxicology. 2021;452:152707. doi:10.1016/j.tox.2021.152707(IF:4.221)
[113] Tao Q, Qi Y, Gu J, et al. Breast cancer cells-derived Von Willebrand Factor promotes VEGF-A-related angiogenesis through PI3K/Akt-miR-205-5p signaling pathway. Toxicol Appl Pharmacol. 2022;440:115927. doi:10.1016/j.taap.2022.115927(IF:4.219)
[114] Li H, Tian J, Yin Y, et al. Interleukin-18 mediated inflammatory brain injury after intracerebral hemorrhage in male mice. J Neurosci Res. 2022;100(6):1359-1369. doi:10.1002/jnr.25044(IF:4.164)
[115] Peng K, Fan X, Li Q, et al. IRF-1 mediates the suppressive effects of mTOR inhibition on arterial endothelium. J Mol Cell Cardiol. 2020;140:30-41. doi:10.1016/j.yjmcc.2020.02.006(IF:4.133)
[116] Zhang X, Yang S, Chen W, et al. Circular RNA circYPEL2: A Novel Biomarker in Cervical Cancer. Genes (Basel). 2021;13(1):38. Published 2021 Dec 23. doi:10.3390/genes13010038(IF:4.096)
[117] Cheng ZY, Hu YH, Xia QP, Wang C, He L. DRD1 agonist A-68930 improves mitochondrial dysfunction and cognitive deficits in a streptozotocin-induced mouse model. Brain Res Bull. 2021;175:136-149. doi:10.1016/j.brainresbull.2021.07.015(IF:4.079)
[118] Wen RX, Shen H, Huang SX, et al. P2Y6 receptor inhibition aggravates ischemic brain injury by reducing microglial phagocytosis. CNS Neurosci Ther. 2020;26(4):416-429. doi:10.1111/cns.13296(IF:4.074)
[119] Zhou MZ, O'Neill Rothenberg D, Zeng W, et al. Discovery and Biochemical Characterization of N-methyltransferase Genes Involved in Purine Alkaloid Biosynthetic Pathway of Camellia gymnogyna Hung T.Chang (Theaceae) from Dayao Mountain. Phytochemistry. 2022;199:113167. doi:10.1016/j.phytochem.2022.113167(IF:4.072)
[120] Sun H, Li Y, Quan X, et al. PIAS3/SOCS1-STAT3 axis responses to oxidative stress in hepatocellular cancer cells. Am J Transl Res. 2021;13(11):12395-12409. Published 2021 Nov 15. (IF:4.060)
[121] Fan J, Zhang X, Jiang Y, Chen L, Sheng M, Chen Y. SPARC knockdown attenuated TGF-β1-induced fibrotic effects through Smad2/3 pathways in human pterygium fibroblasts. Arch Biochem Biophys. 2021;713:109049. doi:10.1016/j.abb.2021.109049(IF:4.013)
[122] Pan C, Liu Q, Wu X. HIF1α/miR-520a-3p/AKT1/mTOR Feedback Promotes The Proliferation And Glycolysis Of Gastric Cancer Cells. Cancer Manag Res. 2019;11:10145-10156. Published 2019 Dec 2. doi:10.2147/CMAR.S223473(IF:3.989)
[123] Xu Q, Luo C, Fu Y, Zhu F. Risk and molecular mechanisms for boscalid resistance in Penicillium digitatum. Pestic Biochem Physiol. 2022;184:105130. doi:10.1016/j.pestbp.2022.105130(IF:3.963)
[124] He W, Xu W, Fu K, Guo W, Kim DS, Zhang J. Positional effects of double-stranded RNAs targeting β-Actin gene affect RNA interference efficiency in Colorado potato beetle. Pestic Biochem Physiol. 2022;184:105121. doi:10.1016/j.pestbp.2022.105121(IF:3.963)
[125] Teng JW, Bian SS, Kong P, Chen YG. Icariin triggers osteogenic differentiation of bone marrow stem cells by up-regulating miR-335-5p. Exp Cell Res. 2022;414(2):113085. doi:10.1016/j.yexcr.2022.113085(IF:3.905)
[126] Wu Q, He Y, Liu X, et al. Cancer stem cell-like cells-derived exosomal CDKN2B-AS1 stabilizes CDKN2B to promote the growth and metastasis of thyroid cancer via TGF-β1/Smad2/3 signaling [published online ahead of print, 2022 Jun 21]. Exp Cell Res. 2022;113268. doi:10.1016/j.yexcr.2022.113268(IF:3.905)
[127] Wu S, Zhang Y, Ma J, et al. Interleukin-6 absence triggers intestinal microbiota dysbiosis and mucosal immunity in mice. Cytokine. 2022;153:155841. doi:10.1016/j.cyto.2022.155841(IF:3.861)
[128] Guo X, Cao X, Fang X, Guo A, Li E. Involvement of phase II enzymes and efflux transporters in the metabolism and absorption of naringin, hesperidin and their aglycones in rats. Int J Food Sci Nutr. 2022;73(4):480-490. doi:10.1080/09637486.2021.2012562(IF:3.833)
[129] Li Z, Zhang X, Yuan T, et al. Addition of Platelet-Rich Plasma to Silk Fibroin Hydrogel Bioprinting for Cartilage Regeneration. Tissue Eng Part A. 2020;26(15-16):886-895. doi:10.1089/ten.TEA.2019.0304(IF:3.776)
[130] Zhao W, Wu M, Cui L, Du W. Limonin attenuates the stemness of cervical carcinoma cells by promoting YAP nuclear-cytoplasmic translocation. Food Chem Toxicol. 2019;125:621-628. doi:10.1016/j.fct.2019.02.011(IF:3.775)
[131] Li X, Yin Y, Fan S, et al. Aspergillus aculeatus enhances potassium uptake and photosynthetic characteristics in perennial ryegrass by increasing potassium availability. J Appl Microbiol. 2022;132(1):483-494. doi:10.1111/jam.15186(IF:3.772)
[132] Su Z, Wang C, Chang D, Zhu X, Sai C, Pei J. Limonin attenuates the stemness of breast cancer cells via suppressing MIR216A methylation. Biomed Pharmacother. 2019;112:108699. doi:10.1016/j.biopha.2019.108699(IF:3.743)
[133] Wang Y, Fang C, Xu L, Yang B, Song E, Song Y. Polybrominated Diphenyl Ether Quinone Exposure Induces Atherosclerosis Progression via CD36-Mediated Lipid Accumulation, NLRP3 Inflammasome Activation, and Pyroptosis. Chem Res Toxicol. 2021;34(9):2125-2134. doi:10.1021/acs.chemrestox.1c00214(IF:3.739)
[134] Shi YJ, Hu SJ, Zhao QQ, Liu XS, Liu C, Wang H. Toll-like receptor 4 (TLR4) deficiency aggravates dextran sulfate sodium (DSS)-induced intestinal injury by down-regulating IL6, CCL2 and CSF3. Ann Transl Med. 2019;7(23):713. doi:10.21037/atm.2019.12.28(IF:3.689)
[135] Shen X, Chen R, Chen X, et al. Molecular evolution and expression analysis of ADP-ribosylation factors (ARFs) from longan embryogenic callus. Gene. 2021;777:145461. doi:10.1016/j.gene.2021.145461(IF:3.688)
[136] Jia Z, Wang J, Chen K, et al. Identification and expression analysis of group II C-type lectin domain containing receptors in grass carp Ctenopharyngodon idella. Gene. 2021;789:145668. doi:10.1016/j.gene.2021.145668(IF:3.688)
[137] Zhao P, Zhang C, Song Y, et al. Genome-wide identification, expression and functional analysis of the core cell cycle gene family during the early somatic embryogenesis of Dimocarpus longan Lour. Gene. 2022;821:146286. doi:10.1016/j.gene.2022.146286(IF:3.688)
[138] Zhang C, Xu X, Xu X, et al. Genome-wide identification, evolution analysis of cytochrome P450 monooxygenase multigene family and their expression patterns during the early somatic embryogenesis in Dimocarpus longan Lour. Gene. 2022;826:146453. doi:10.1016/j.gene.2022.146453(IF:3.688)
[139] Wang M, Tao H, Huang P. Clinical significance of LARGE1 in progression of liver cancer and the underlying mechanism. Gene. 2021;779:145493. doi:10.1016/j.gene.2021.145493(IF:3.688)
[140] Chen X, Xu X, Shen X, et al. Genome-wide investigation of DNA methylation dynamics reveals a critical role of DNA demethylation during the early somatic embryogenesis of Dimocarpus longan Lour. Tree Physiol. 2020;40(12):1807-1826. doi:10.1093/treephys/tpaa097(IF:3.655)
[141] Mao MG, Xu J, Liu RT, Ye L, Wang R, Jiang JL. Fas/FasL of pacific cod mediated apoptosis. Dev Comp Immunol. 2021;119:104022. doi:10.1016/j.dci.2021.104022(IF:3.636)
[142] Gu D, Zhou X, Ma Y, et al. Expression of a Brassica napus metal transport protein (BnMTP3) in Arabidopsis thaliana confers tolerance to Zn and Mn. Plant Sci. 2021;304:110754. doi:10.1016/j.plantsci.2020.110754(IF:3.591)
[143] Fu Y, Wang X, Zhang L, Ren Y, Hao L. Allograft inflammatory factor-1 enhances inflammation and oxidative stress via the NF-κB pathway in diabetic kidney disease. Biochem Biophys Res Commun. 2022;614:63-69. doi:10.1016/j.bbrc.2022.04.089(IF:3.575)
[144] Meng H, Shang Y, Cheng Y, et al. Knockout of zebrafish colony-stimulating factor 1 receptor by CRISPR/Cas9 affects metabolism and locomotion capacity. Biochem Biophys Res Commun. 2021;551:93-99. doi:10.1016/j.bbrc.2021.02.122(IF:3.575)
[145] Chen Y, Cao A, Li Q, Quan J. Identification of DNA aptamers that specifically targets EBV+ nasopharyngeal carcinoma via binding with EphA2/CD98hc complex. Biochem Biophys Res Commun. 2022;608:135-141. doi:10.1016/j.bbrc.2022.03.157(IF:3.575)
[146] Li M, Ning N, Liu Y, et al. The potential of Zishen Yutai pills to facilitate endometrial recovery and restore fertility after induced abortion in rats. Pharm Biol. 2021;59(1):1505-1516. doi:10.1080/13880209.2021.1993272(IF:3.503)
[147] Liu T, Shao Q, Wang W, et al. Integrating network pharmacology and experimental validation to decipher the mechanism of the Chinese herbal prescription JieZe-1 in protecting against HSV-2 infection. Pharm Biol. 2022;60(1):451-466. doi:10.1080/13880209.2022.2038209(IF:3.503)
[148] Song H, Xu Y, Shi L, et al. LncRNA THOR increases the stemness of gastric cancer cells via enhancing SOX9 mRNA stability. Biomed Pharmacother. 2018;108:338-346. doi:10.1016/j.biopha.2018.09.057(IF:3.457)
[149] Zhang Y, Zhu Z, Zhai W, Bi Y, Yin Y, Zhang W. Expression and purification of asprosin in Pichia pastoris and investigation of its increase glucose uptake activity in skeletal muscle through activation of AMPK. Enzyme Microb Technol. 2021;144:109737. doi:10.1016/j.enzmictec.2020.109737(IF:3.448)
[150] Tao X, Mo L, Zeng L. Hyperoxia Induced Bronchopulmonary Dysplasia-Like Inflammation via miR34a-TNIP2-IL-1β Pathway. Front Pediatr. 2022;10:805860. Published 2022 Mar 30. doi:10.3389/fped.2022.805860(IF:3.418)
[151] Li Y, Wu M, Xu S, Huang H, Yan L, Gu Y. Colorectal cancer stem cell-derived exosomal long intergenic noncoding RNA 01315 (LINC01315) promotes proliferation, migration, and stemness of colorectal cancer cells. Bioengineered. 2022;13(4):10827-10842. doi:10.1080/21655979.2022.2065800(IF:3.269)
[152] Kong L, Li J, Yang Y, Tang H, Zou H. Paeoniflorin alleviates the progression of retinal vein occlusion via inhibiting hypoxia inducible factor-1α/vascular endothelial growth factor/STAT3 pathway. Bioengineered. 2022;13(5):13622-13631. doi:10.1080/21655979.2022.2081755(IF:3.269)
[153] Shi P, Liu Y, Yang D, et al. CircRNA ZNF609 promotes the growth and metastasis of thyroid cancer in vivo and in vitro by downregulating miR-514a-5p. Bioengineered. 2022;13(2):4372-4384. doi:10.1080/21655979.2022.2033015(IF:3.269)
[154] Fang M, Li Y, Wu Y, Ning Z, Wang X, Li X. miR-185 silencing promotes the progression of atherosclerosis via targeting stromal interaction molecule 1. Cell Cycle. 2019;18(6-7):682-695. doi:10.1080/15384101.2019.1580493(IF:3.259)
[155] Wang J, Liu Y, Yin R, Wang N, Xiao T, Hirai H. RNA-Seq analysis of Phanerochaete sordida YK-624 degrades neonicotinoid pesticide acetamiprid [published online ahead of print, 2022 Jan 20]. Environ Technol. 2022;1-8. doi:10.1080/09593330.2022.2026488(IF:3.247)
[156] Qin Y, Sun Z, Wang W, et al. Characterization of CD3γ/δ+ cells in grass carp (Ctenopharyngodon idella). Dev Comp Immunol. 2021;114:103791. doi:10.1016/j.dci.2020.103791(IF:3.192)
[157] Gong F, Dong D, Zhang T, Xu W. Long non-coding RNA FENDRR attenuates the stemness of non-small cell lung cancer cells via decreasing multidrug resistance gene 1 (MDR1) expression through competitively binding with RNA binding protein HuR. Eur J Pharmacol. 2019;853:345-352. doi:10.1016/j.ejphar.2019.04.022(IF:3.170)
[158] Song H, Shi L, Xu Y, et al. BRD4 promotes the stemness of gastric cancer cells via attenuating miR-216a-3p-mediated inhibition of Wnt/β-catenin signaling. Eur J Pharmacol. 2019;852:189-197. doi:10.1016/j.ejphar.2019.03.018(IF:3.170)
[159] Xue Y, Yu X, Zhang X, et al. Protective effects of ginsenoside Rc against acute cold exposure-induced myocardial injury in rats. J Food Sci. 2021;86(7):3252-3264. doi:10.1111/1750-3841.15757(IF:3.167)
[160] Hei X, Xie M, Xu J, Li J, Liu T. β-Asarone Exerts Antioxidative Effects on H2O2-Stimulated PC12 Cells by Activating Nrf2/HO-1 Pathway. Neurochem Res. 2020;45(8):1953-1961. doi:10.1007/s11064-020-03060-9(IF:3.038)
[161] Ye C, Xu S, Hu Q, et al. Structure and function analysis of various brain subregions and pituitary in grass carp (Ctenopharyngodon idellus). Comp Biochem Physiol Part D Genomics Proteomics. 2020;33:100653. doi:10.1016/j.cbd.2019.100653(IF:3.011)
[162] Wang WJ, Jiang X, Gao CC, Chen ZW. Salusin‑β participates in high glucose‑induced HK‑2 cell ferroptosis in a Nrf‑2‑dependent manner. Mol Med Rep. 2021;24(3):674. doi:10.3892/mmr.2021.12313(IF:2.952)
[163] Liu Z, Li J, Hu X, Xu H. Helicobacter pylori-induced protein tyrosine phosphatase receptor type C as a prognostic biomarker for gastric cancer. J Gastrointest Oncol. 2021;12(3):1058-1073. doi:10.21037/jgo-21-305(IF:2.892)
[164] Wu H, Huang S, Zhuang W, Qiao G. Prognostic significance of eight immune-related genes on survival in patients with lung squamous cell carcinoma. Biomark Med. 2021;15(4):295-306. doi:10.2217/bmm-2020-0483(IF:2.851)
[165] Wu H, Yue S, Huang Y, Zhao X, Cao H, Liao M. Genome-Wide Identification of the Long Noncoding RNAs of Tribolium castaneum in Response to Terpinen-4-ol Fumigation. Insects. 2022;13(3):283. Published 2022 Mar 14. doi:10.3390/insects13030283(IF:2.769)
[166] Zhao F, Wang X, Li Y, Chen X, Geng Z, Zhang C. Effects of Dietary Supplementation with Epigallocatechin Gallate on Meat Quality and Muscle Antioxidant Capacity of Broilers Subjected to Acute Heat Stress. Animals (Basel). 2021;11(11):3296. Published 2021 Nov 18. doi:10.3390/ani11113296(IF:2.752)
[167] Peng J, Wang Q, Meng Z, et al. A loss-of-function mutation p.T256M in NDRG4 is implicated in the pathogenesis of pulmonary atresia with ventricular septal defect (PA/VSD) and tetralogy of Fallot (TOF). FEBS Open Bio. 2021;11(2):375-385. doi:10.1002/2211-5463.13044(IF:2.693)
[168] Ma S, Feng X, Liu F, Wang B, Zhang H, Niu X. The pro-inflammatory response of macrophages regulated by acid degradation products of poly(lactide-co-glycolide) nanoparticles. Eng Life Sci. 2021;21(10):709-720. Published 2021 May 12. doi:10.1002/elsc.202100040(IF:2.678)
[169] Huang R, Dong R, Wang N, Lan B, Zhao H, Gao Y. Exploring the Antiglioma Mechanisms of Luteolin Based on Network Pharmacology and Experimental Verification. Evid Based Complement Alternat Med. 2021;2021:7765658. Published 2021 Nov 27. doi:10.1155/2021/7765658(IF:2.630)
[170] Guan J, Qin Y, Deng G, Zhao H. GOLM1 as a Potential Therapeutic Target Modulates B7-H3 Secretion to Drive Ovarian Cancer Metastasis. Evid Based Complement Alternat Med. 2022;2022:5151065. Published 2022 Jan 25. doi:10.1155/2022/5151065(IF:2.630)
[171] Zhang H, Yu W, Ji L, et al. Guifu Dihuang Pills Ameliorated Mucus Hypersecretion by Suppressing Muc5ac Expression and Inactivating the ERK-SP1 Pathway in Lipopolysaccharide/Cigarette Smoke-Induced Mice. Evid Based Complement Alternat Med. 2021;2021:9539218. Published 2021 Nov 3. doi:10.1155/2021/9539218(IF:2.630)
[172] Huang Q, Li CY, Zhang N, et al. The Effects of Moxibustion on Learning and Memory and m6A RNA Methylation in APP/PS1 Mice. Evid Based Complement Alternat Med. 2022;2022:2998301. Published 2022 Mar 21. doi:10.1155/2022/2998301(IF:2.630)
[173] Li Y, Zhang J, Pan S, Zhou J, Diao X, Liu S. CircRNA CDR1as knockdown inhibits progression of non-small-cell lung cancer by regulating miR-219a-5p/SOX5 axis. Thorac Cancer. 2020;11(3):537-548. doi:10.1111/1759-7714.13274(IF:2.610)
[174] Xiao L, Zhang S, Zheng Q, Zhang S. Dysregulation of KIF14 regulates the cell cycle and predicts poor prognosis in cervical cancer: a study based on integrated approaches. Braz J Med Biol Res. 2021;54(11):e11363. Published 2021 Sep 3. doi:10.1590/1414-431X2021e11363(IF:2.590)
[175] Hu Q, Lan J, Liang W, et al. MMP7 damages the integrity of the renal tubule epithelium by activating MMP2/9 during ischemia-reperfusion injury. J Mol Histol. 2020;51(6):685-700. doi:10.1007/s10735-020-09914-4(IF:2.531)
[176] Zhu J, Guo C, Lu P, Shao S, Tu B. Contribution of Growth Arrest-Specific 5/miR-674 to the Hypothalamus Pituitary Adrenal Axis Regulation Effect by Electroacupuncture following Trauma. Neuroimmunomodulation. 2021;28(3):137-149. doi:10.1159/000513385(IF:2.492)
[177] Xue Y, Fu W, Liu Y, et al. Ginsenoside Rb2 alleviates myocardial ischemia/reperfusion injury in rats through SIRT1 activation. J Food Sci. 2020;85(11):4039-4049. doi:10.1111/1750-3841.15505(IF:2.479)
[178] Yu X, Jiang N, Li J, Li X, He S. Upregulation of BRD7 protects podocytes against high glucose-induced apoptosis by enhancing Nrf2 in a GSK-3β-dependent manner. Tissue Cell. 2022;76:101813. doi:10.1016/j.tice.2022.101813(IF:2.466)
[179] Cheng YY, Yang X, Gao X, Song SX, Yang MF, Xie FM. LGR6 promotes glioblastoma malignancy and chemoresistance by activating the Akt signaling pathway. Exp Ther Med. 2021;22(6):1364. doi:10.3892/etm.2021.10798(IF:2.447)
[180] Hu F, Rao M, Zhang M, et al. Long non-coding RNA profiles in plasma exosomes of patients with gastric high-grade intraepithelial neoplasia. Exp Ther Med. 2022;23(1):1. doi:10.3892/etm.2021.10923(IF:2.447)
[181] Zhou W, Huang K, Zhang Q, et al. LINC00844 promotes proliferation and migration of hepatocellular carcinoma by regulating NDRG1 expression. PeerJ. 2020;8:e8394. Published 2020 Jan 28. doi:10.7717/peerj.8394(IF:2.379)
[182] Li M, Xia S, Shi P. DPM1 expression as a potential prognostic tumor marker in hepatocellular carcinoma. PeerJ. 2020;8:e10307. Published 2020 Nov 24. doi:10.7717/peerj.10307(IF:2.379)
[183] Li L, Dong L, Zhao J, et al. Circulating miRNA-3552 as a Potential Biomarker for Ischemic Stroke in Rats. Biomed Res Int. 2020;2020:4501393. Published 2020 Jul 16. doi:10.1155/2020/4501393(IF:2.276)
[184] Zhou JP, Ren YD, Xu QY, et al. Obesity-Induced Upregulation of ZBTB7A Promotes Lipid Accumulation through SREBP1. Biomed Res Int. 2020;2020:4087928. Published 2020 Jan 7. doi:10.1155/2020/4087928(IF:2.276)
[185] Lin C, Sun L, Huang S, Weng X, Wu Z. STC2 Is a Potential Prognostic Biomarker for Pancreatic Cancer and Promotes Migration and Invasion by Inducing Epithelial-Mesenchymal Transition. Biomed Res Int. 2019;2019:8042489. Published 2019 Jul 15. doi:10.1155/2019/8042489(IF:2.197)
[186] Li Y, Li B, Liu Y, et al. Porphyromonas gingivalis lipopolysaccharide affects oral epithelial connections via pyroptosis. J Dent Sci. 2021;16(4):1255-1263. doi:10.1016/j.jds.2021.01.003(IF:2.080)
[187] Shang J, Chen WM, Liu S, et al. CircPAN3 contributes to drug resistance in acute myeloid leukemia through regulation of autophagy. Leuk Res. 2019;85:106198. doi:10.1016/j.leukres.2019.106198(IF:2.066)
[188] Zang Y, Zhu L, Li T, et al. EI24 Suppresses Tumorigenesis in Pancreatic Cancer via Regulating c-Myc. Gastroenterol Res Pract. 2018;2018:2626545. Published 2018 Oct 2. doi:10.1155/2018/2626545(IF:1.859)
[189] Xu LL, Shang Y, Qu XN, He HM. M-Phase Phosphoprotein 9 upregulation associates with poor prognosis and activates mTOR signaling in gastric cancer. Kaohsiung J Med Sci. 2021;37(3):208-214. doi:10.1002/kjm2.12319(IF:1.737)
[190] Lv J, Liu W, Shi G, et al. Human cardiac extracellular matrix-chitosan-gelatin composite scaffold and its endothelialization. Exp Ther Med. 2020;19(2):1225-1234. doi:10.3892/etm.2019.8349(IF:1.448)
[191] Yang L, Tong Y, Chen PF, Miao S, Zhou RY. Neuroprotection of dihydrotestosterone via suppression of the toll-like receptor 4/nuclear factor-kappa B signaling pathway in high glucose-induced BV-2 microglia inflammatory responses. Neuroreport. 2020;31(2):139-147. doi:10.1097/WNR.0000000000001385(IF:1.394)
[192] Liao Y, Zhao T, Li LY, Wang FQ. Elevated Sad1 and UNC84 Domain Containing 2 (SUN2) level inhibits cell growth and aerobic glycolysis in oral cancer through reducing the expressions of glucose transporter 1 (GLUT1) and lactate dehydrogenase A (LDHA). J Dent Sci. 2021;16(1):460-466. doi:10.1016/j.jds.2020.08.007(IF:1.034)
[193] Jiang F, Tang XW, Li J, Zhou JY, Zuo LD, Li DZ. Hb Lepore-Hong Kong: First Report of a Novel δ/β-Globin Gene Fusion in a Chinese Family. Hemoglobin. 2021;45(4):220-224. doi:10.1080/03630269.2021.1956945(IF:0.849)
