Q：Hifair® Ⅲ SuperMix 后续只能用于 qPCR 实验吗？

A：推荐用于qPCR 实验，常规长度基因克隆实验也可以用。

Q：为什么说Hifair® Ⅲ SuperMix 操作误差更小，重复性更好？

A：Hifair® Ⅲ SuperMix 只有 3 个组分，且每个组分的添加体积均超过 1μL，因此移液误差更小，重复性更好。

Q：Hifair® Ⅲ SuperMix 逆转录反应的温度应设置为多少？

A：常规模板 55℃即可，如果是具有复杂二级结构的模板或高 GC 含量的模板，可将温度适当提高至 65℃，有利于提高逆转录效率。

Q：Hifair® Ⅲ SuperMix 操作时可以不进行gDNA 去除这一步吗？

A：可以省略。

Q：为什么 Hifair® Ⅲ SuperMix 更有利于低浓度模板检测？

A：一是该产品增强了与模板的亲和力，二是由于组分的设置，使得 RNA 最大投入量可以达到 12μL，对于低浓度模板可通过增大投入体积来增大模板投入量。

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