细胞转染实验从开始设计到最终的效果检测，可以由6个过程组成：核酸准备、试剂选择、细胞铺板、转染操作、细胞培养、效果检测。每一步都会影响最终的结果，均存在不同的注意事项。上个月小翌分享了前3个过程的注意事项：
本期分享后面3个过程的注意事项：
转染操作
1.条件摸索与优化：
2.无血清配置复合物：
3.孵育时间：
4.抗生素影响：
1.优良的培养试剂：
2.是否换液：
1.检测方法的选择：
2.检测时间的选择：
3.稳转筛选：
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应用场景 | 名称 | 货号 | 规格 | 价格（元） |
细胞类型：贴壁/悬浮 | 40802ES01 | 100 μL | 168.00 | |
40802ES02 | 0.5 mL | 738.00 | ||
40802ES03 | 1.0 mL | 1308.00 | ||
40802ES08 | 5×1mL | 4878.00 | ||
细胞类型：贴壁/悬浮 核酸类型：DNA | 40803ES70 | 200 T | 625.00 | |
用途：病毒感染+DNA转染 | 40804ES76 | 500 μL | 180.00 | |
40804ES86 | 5×500 μL | 500.00 | ||
细胞类型：悬浮 | Hieff TransTM Suspension Cell-Free Liposomal Transfection Reagent 悬浮细胞专用脂质体核酸转染试剂 | 40805ES01 | 100 μL | 228.00 |
40805ES02 | 0.5 mL | 948.00 | ||
40805ES03 | 1.0 mL | 1678.00 | ||
40805ES08 | 5×1 mL | 5268.00 | ||
细胞类型：贴壁/悬浮 核酸类型：siRNA、miRNA | Hieff TransTM in vitro siRNA/miRNA Transfection Reagent siRNA/miRNA体外转染试剂 | 40806ES01 | 0.1 mL | 372.00 |
40806ES02 | 0.5 mL | 1472.00 | ||
40806ES03 | 1.0 mL | 2572.00 | ||
细胞类型：贴壁/悬浮 核酸类型：DNA、siRNA、miRNA | Hieff TransTM Universal Transfection Reagent Hieff TransTM通用型转染试剂 | 40808ES02 | 0.5 mL | 1165.00 |
40808ES03 | 1 mL | 2065.00 | ||
40808ES08 | 5×1 mL | 7985.00 | ||
细胞类型：贴壁/悬浮 核酸类型：DNA | 40815ES03 | 1 g | 1855.00 | |
40815ES08 | 5×1 g | 7255.00 | ||
细胞类型：贴壁/悬浮 核酸类型：DNA | Polyethylenimine Linear(PEI) MW40000（rapid lysis）线性PEI转染试剂（速溶型）MW40000 | 40816ES02 | 100 mg | 655.00 |
40816ES03 | 1 g | 1855.00 |
