Yeasen胎牛血清（特级），经3次100 nm过滤和支原体、病毒筛查，经过严格检验测试后全自动化罐装，规范化生产，品质保证，含有丰富的细胞生长所需的营养成分，适用于培养大部分常规细胞系。

产品选购指南

1. 适用于绝大多数常规细胞的培养，如常规肿瘤细胞、规模化生产细胞（293T、CHO、VERO等）、免疫细胞（RAW264.7、THP-1等）。其他娇贵细胞或难培养的细胞（如干细胞（如NSC、PB-MSCs等）、难培养的原代细胞（上皮细胞、内皮细胞、心肌细胞等）、其他难培养的细胞（如部分乳腺癌细胞、部分结直肠癌细胞、部分肺腺癌细胞、部分胶质瘤细胞等）建议选用40131ES、40132ES。

2. 多种生长因子，细胞培养状态稳定。

3. 纯天然制品，不含任何人为添加成分。

4. 为避免反复冻融，可以选购小规格（50 mL）产品，即用即取，方便快捷。10个50 mL产品可等同于500 mL产品。

产品优势

1. 兼容范围广——适用于多种常规细胞培养

2. 产品纯天然——对标进口胎牛血清，营养丰富，不含任何人为添加成分

3. 细胞状态好——多种生长因子，批次稳定，细胞培养状态稳定

4. 小规格便利——50 mL便携装，即用即取，避免反复冻融，避免沉淀产生，避免分装污染，方便快捷，产品升级不加价

5. 产品质控严——经过三次0.1 μM无菌过滤，有效去除微生物，通过支原体和病毒筛查检测

6. 内毒素极低——内毒素≤5 EU/mL，内毒素含量低，完全符合国家质量标准

7. 客户群体广——使用课题组数多达4000+，如中科院上海生命科学研究院，清华大学，浙江大学，上海交通大学等各大高校和研究院

8. 发表文献多——客户发表的文章引用影响因子累计高达1000+

产品应用案例（细胞培养）

产品应用案例（单克隆实验）

客户使用本产品发表的科研文献（部分）

[1] S. Zou, Q. Zhang, M., et al. Radiofrequency Dynamic Therapy on Cancer of Gallium–Indium Liquid Metal Nanoemulsion Stabilized by Gold Nanoclusters. Adv. Funct. Mater. 2025, 2425330. (IF:18.5)

[2] Deng J, Wang Z, Wu L, et al. Metal-Phenolic Network Hydrogel Vaccine Platform for Enhanced Humoral Immunity against Lethal Rabies Virus. ACS Nano. 2025 Mar 11;19(9):9042-9052. doi: 10.1021/acsnano.4c17759. Epub 2025 Mar 2. PMID: 40025824. (IF:15.8)

[3] Hu S, Peng L, , et al. SPT5 stabilizes RNA polymerase II, orchestrates transcription cycles, and maintains the enhancer landscape. Mol Cell. 2021;81(21):4425-4439.e6. doi:10.1016/j.molcel.2021.08.029(IF:17.970)

[4] Tu J, Li W, Yang S, et al. Single-Cell Transcriptome Profiling Reveals Multicellular Ecosystem of Nucleus Pulposus during Degeneration Progression. Adv Sci (Weinh). 2022;9(3):e2103631. doi:10.1002/advs.202103631(IF:16.806)

[5] Zhang W, Liu J, Li X, et al. Precise Chemodynamic Therapy of Cancer by Trifunctional Bacterium-Based Nanozymes. ACS Nano. 2021;15(12):19321-19333. doi:10.1021/acsnano.1c05605(IF:15.881)

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[7] Cai Z, Zhang Y, Zhang W, et al. Arsenic retention in erythrocytes and excessive erythrophagocytosis is related to low selenium status by impaired redox homeostasis. Redox Biol. 2022;52:102321. doi:10.1016/j.redox.2022.102321(IF:11.799)

[8] Li S, Zhang J, Qian S, et al. S100A8 promotes epithelial-mesenchymal transition and metastasis under TGF-β/USF2 axis in colorectal cancer. Cancer Commun (Lond). 2021;41(2):154-170. doi:10.1002/cac2.12130(IF:10.392)

客户使用本产品的数据展示

产品应用

运输方式：干冰运输；

保存方式：-20℃至-10℃可保存5年。

【注】一旦解冻，血清应该保存在2℃到8℃冰箱，存储时间不宜超过6周。如果需要长期保存，建议将血清在无菌环境下先进行分装，后重新冷冻保存，避免反复冻融。使用过程中，避免反复冻融，避免紫外线照射。

解冻的方式（二选一）

1) 将血清从-20℃存储条件下取出，放置于2-8℃冰箱中过夜，使其部分溶解，然后在室温条件下使其全部融解，溶解过程中须不时的摇动瓶身混合里面的液体。建议采取此种方式解冻更优。

2) 直接将血清从-20℃取出后，放置于37℃水浴中，不断摇晃瓶身使其加快解冻和混合。解冻以后不要在37℃水浴放置太长时间。

【注】如果不晃动瓶身，当温度超过40℃的时候沉积在瓶底的物质有可能会发生蛋白变形，出现沉淀。如果出现沉淀，建议可将血清分装到无菌离心管中，400~600 g（如500 g）离心5 min，取血清上清液，加入到基础培养基中，再一起过滤，全程保证无菌环境即可。

Q: 请问我们的血清拿到之后还需要热灭活吗？

A: 本司产品出厂之前都经过了灭活处理，所以一般情况下，就不需要再操作一次灭活了。

Q: 解冻后血清中有悬浮物质/絮状沉淀，因怎样处理？

A: 血清中沉淀物的出现有许多种原因，但最普遍的原因是由于血清中脂蛋白的变性所造成,而血纤维蛋白(形成凝血的蛋白之一)在血清解冻后,也会存在于血清中,亦是造成沉淀物的主要原因之一。但这些絮状沉淀物,并不影响血清本身的质量。

去除这些絮状沉淀物,可以将血清分装至无菌离心管内,以400g稍微离心10-15mins去除沉淀。我们不建议以过滤的方法去除这些絮状物,因为它可能会阻塞您过滤膜。建议在使用血清的时候,注意正确的血清解冻步骤,并尽量避免灭活血清及长时间的将血清置于高温环境中。

Q：为什么本司血清看着比较黄，竞品公司血清比较红？

A: 血红蛋白的原因，血红蛋白含量比较高，血清就会比较红，血红蛋白含量较少，所以会比较黄，血清本质是黄的。

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