DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily. Mature human DNAM-1 contains a 236 amino acid (aa) extracellular domain (ECD) with two Ig-like C2-set domains and a 61 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins. Within the ECD, human DNAM-1 shares 50% and 52% aa sequence identity with mouse and rat DNAM-1, respectively. DNAM-1 is expressed on multiple lymphoid and myeloid cells and interacts with CD155/PVR and Nectin-2/CD112. Ligation of DNAM-1 promotes the activation of NK cells, CD8+ T cells, and mast cells, dendritic cell maturation, megakaryocyte and activated platelet adhesion to vascular endothelial cells, and monocyte extravasation; it inhibits the forrmation of osteoclasts. Platelet-endothelium interactions mediated by DNAM-1 enable the metastasis of tumor cells to the lung. In activated, but not in resting NK, T, and mast cells, the cis association of DNAM-1 with CD18 contributes to the tyrosine and serine phosphorylation of DNAM-1 during activation.

 

产品性质

别名（Synonyms）	CD226 molecule; CD226; DNAM1; PTA1; TLiSA1
Uniprot No.	Q15762
表达区间及表达系统（Source）	Recombinant Human DNAM-1/CD226 Protein is expressed from HEK293 Cells with hFc tag at the C-terminal. It contains Glu19-Asn247.
分子量(Molecular Weight)	The protein has a predicted MW of 52.8 kDa. Due to glycosylation, the protein migrates to 70-100 kDa based on Tris-Bis PAGE result.
外观(Physical Appearance）	Sterile Filtered White lyophilized (freeze-dried) powder.
纯度（Purity）	> 95% as determined by SDS-PAGE and HPLC
活性（Activity）	1.ELISA Data：Immobilized Human CD155, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human DNAM-1/CD226, hFc Tag with the EC50 of 1.8μg/ml determined by ELISA. 2.SPR Data：Human DNAM-1, hFc Tag captured on CM5 Chip via Protein A can bind Rhesus macaque CD155, His Tag with an affinity constant of 0.234 μM as determined in SPR assay
内毒素（Endotoxin）	< 1.0 EU per 1μg of the protein by the LAL method.
制剂（Formulation）	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
复溶（Reconstitution）	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

 

运输与保存方法

冰袋运输。-20℃至-80℃保存，一年有效期。

复溶后，-20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。

建议第一次使用时分装冻存，避免反复冻融。

 

注意事项

1. 避免反复冻融。

2. 为了您的安全和健康，请穿实验服并戴一次性手套操作。

3. 本产品仅作科研用途！

 

产品数据

Tris-Bis PAGE

HB220624