产品说明书
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产品信息
|
产品名称 |
产品编号 |
规格 |
|
Recombinant Human 4-1BB/TNFRSF9 (His Tag) |
93544ES25 |
25 μg |
|
93544ES60 |
100 μg |
|
|
93544ES76 |
500 μg |
|
|
93544ES80 |
1 mg |
产品描述
4-1BB, is also known as CD137, is a type 2 transmembrane glycoprotein receptor belonging to the TNF superfamily. CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. In addition, CD137 expression is found on dendritic cells, B cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation.
产品性质
|
别名（Synonyms） |
CD137; TNFRSF9; 4-1BB ligand receptor; 41BB; 4-1BB; CDw137; FLJ43501; ILA; T cell antigen ILA |
|
Uniprot No. |
Q07011 |
|
表达区间及表达系统（Source） |
HEK293 Cells-derived Human 4-1BB/TNFRSF9 Protein, Leu24-Gln186 with His tag at the C-terminal |
|
分子量(Molecular Weight) |
Approximately 18.1 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result. |
|
外观(Physical Appearance） |
Sterile Filtered White lyophilized (freeze-dried) powder. |
|
纯度（Purity） |
> 95% as determined by SDS-PAGE and HPLC |
|
活性（Activity） |
ELISA Data: Immobilized Human 4-1BB, His Tag at 1μg/mL (100 μL/Well) on the plate. Dose response curve for Human 4-1BB Ligand Trimer, hFc Tag with the EC50 of 5.7 ng/mL determined by ELISA. |
|
内毒素（Endotoxin） |
< 1.0 EU per 1μg of the protein by the LAL method. |
|
制剂（Formulation） |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
|
复溶（Reconstitution） |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
运输与保存方法
冰袋运输。-20℃至-80℃保存，一年有效期。
复溶后，-20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。
建议第一次使用时分装冻存，避免反复冻融。
注意事项
- 避免反复冻融。
- 为了您的安全和健康，请穿实验服并戴一次性手套操作。
- 本产品仅作科研用途！
产品数据
Tris-Bis PAGE
SEC-HPLC
ELISA Data
HB240312
