产品说明书
FAQ
COA
已发表文献
PD-1 a receptor expressed on T cells responsible for modulating T cell activation. PD-1 is classified as an immune checkpoint inhibitory receptor. PD1 is expressed on activated T cells, B cells, monocytes, and dendritic cells while PD-L1 expression is constitutive on the same cells and also on nonhematopoietic cells such as lung endothelial cells and hepatocytes. Ligation of PD-L1 with PD-1 induces co-inhibitory signals on T cells promoting their apoptosis, anergy, and functional exhaustion.
产品性质
|
别名 |
PDCD1; PD1; CD279; SLEB2; PD-1; Programmed Death-1 receptor |
|
Uniprot No. |
Q15116.1 |
|
表达区间及表达系统 |
Recombinant Biotinylated Human PD-1/PDCD1 Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Leu25-Gln167. |
|
分子量 |
Approximately 18.9 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. |
|
纯度 |
> 95% as determined by SDS-PAGE and HPLC. |
|
活性 |
ELISA Data: Immobilized Biotinylated Human PD-1, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-PD-1 Antibody, hFc Tag with the EC50 of 14.0ng/ml determined by ELISA. SPR Data: Human PD-L1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human PD-1, His-Avi Tag with an affinity constant of 2.356 μM as determined in SPR assay (Biacore T200). |
|
内毒素 |
< 1.0 EU per 1μg of the protein by the LAL method. |
|
制剂 |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
复溶 |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
运输与保存方法
冰袋运输。-20℃保存，一年有效期。
复溶后，-20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。
建议第一次使用时分装冻存，避免反复冻融。
注意事项
1.为了您的安全和健康，请穿实验服并戴一次性手套操作。
2.本产品仅作科研用途！
产品数据
HB221204
