CD160 (also Natural killer cell receptor BY55) is a 27-30 kDa member of the Ig superfamily. In human, it is expressed principally on nonmyeloid hematopoietic cells. These include CD56DIMCD16⁺ cytolytic NK cells, CD8⁺CD28^- T cells, CD8⁺CD101⁺ IELs, NKT cells, gamma δ TCR T cells, activated CD4⁺ T cells, and vascular endothelial cells. CD160 was initially identified as a GPI‑linked glycoprotein. It is synthesized as a preproprotein that is 181 amino acids (aa) in length. The precursor contains a 26 aa signal sequence, a 133 aa mature molecule that shows one 96 aa V-type Ig-like domain (aa 27-122), and a 22 aa prosegment that is cleaved to generate a GPI-linkage at Ser159. GPI-linked CD160 is known to be cleaved by phospholipases and these generate an 80 kDa (presumably trimeric) band in SDS-PAGE. Alternative splice forms for CD160 are reported to exist on activated NK cells. The principal variant is an extended type I transmembrane (TM) protein that shows a 55 aa substitution for the C-terminal two amino acids. It contains a 23 aa TM segment (aa 160-182) and a 52 aa cytoplasmic region. Two other variants show deletions of the Ig-like domain in both the GPI-linked and TM form .

 

产品性质

别名	CD160; BY55; BY55FLJ46513; NK1; NK28
Uniprot No.	O95971
表达区间及表达系统	Recombinant Human CD160 Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Gly25-Leu158.
分子量	Approximately 17.7 kDa. Due to glycosylation, the protein migrates to 27-33 kDa based on Tris-Bis PAGE result.
纯度	> 95% as determined by SDS-PAGE and HPLC.
活性	SPR Data: Human HVEM captured on Protein A chip, can bind Human CD160, His Tag with an affinity constant of 1.2μM as determined in a SPR assay (Biacore T200).
内毒素	< 1.0 EU per 1μg of the protein by the LAL method.
制剂	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
复溶	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

 

运输与保存方法

冰袋运输。-20℃至-80℃保存，一年有效期。

复溶后，-20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。

建议第一次使用时分装冻存，避免反复冻融。

 

注意事项

1. 避免反复冻融。

2. 为了您的安全和健康，请穿实验服并戴一次性手套操作。

3. 本产品仅作科研用途！

 

产品数据
 

HB220805