产品说明书
FAQ
COA
已发表文献
LAG-3, is a protein which in humans is encoded by the LAG3 gene, which is a cell surface molecule with diverse biologic effects on T cell function. LAG-3 shares approximately 20% amino acid sequence homology with CD4, but has similar structure and binds to MHC class II with higher affinity, providing negative regulation of T cell receptor signaling. Human LAG-3 shares 70%, 67%, 76%, and 73% aa sequence identity with mouse, rat, porcine, and bovine LAG-3, respectively. LAG-3 is expressed on activated CD4+ and CD8+ T cells, NK cells, and plasmacytoid dendritic cells (pDC), but not on resting T cells. LAG-3 on activated CD4+CD25+ Treg cells plays a role in their suppressive activity. LAG-3 limits the expansion of activated T cells and pDC in response to selected stimuli.
产品性质
|
别名（Synonyms） |
CD223, FDC, LAG3, LAG-3, sLAG-3 |
|
Uniprot No. |
P18627-1 |
|
表达区间及表达系统（Source） |
HEK293 Cells-derived Human LAG3 / CD223 Protein, Leu23-Gly434 with His tag at the C-terminal |
|
分子量(Molecular Weight) |
Approximately 45.5 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result. |
|
外观(Physical Appearance） |
Sterile Filtered White lyophilized (freeze-dried) powder. |
|
纯度（Purity） |
> 95% as determined by SDS-PAGE and HPLC |
|
活性（Activity） |
1.ELISA Data: Immobilized Human LAG3, His Tag at 0.5 μg/ml (100 μL/Well). Dose response curve for Anti-LAG3 Ab., hFc Tag with the EC50 of 0.17 μg/mL determined by ELISA. 2.ELISA Data: Immobilized Human LAG3, His Tag at 1μg/mL (100 μL/Well) on the plate. Dose response curve for Human FGL1, hFc Tag with the EC50 of 2.3 μg/mL determined by ELISA. |
|
内毒素（Endotoxin） |
< 1.0 EU per 1μg of the protein by the LAL method. |
|
制剂（Formulation） |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
|
复溶（Reconstitution） |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
运输与保存方法
冰袋运输。-20℃至-80℃保存，一年有效期。
复溶后，-20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。
建议第一次使用时分装冻存，避免反复冻融。
注意事项
1. 避免反复冻融。
2. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
3. 本产品仅作科研用途！
产品数据
HB220628
