Name: 双模式RNA建库试剂盒(兼容Illumina和MGI) Hieff NGS® Ultima Dual-mode RNA Library Prep Kit（12308ES）
Brand: 翌圣生物
SKU: 12308ES
Price: 1790 CNY
Availability: InStock

Hieff NGS® Ultima Dual-mode RNA Library Prep Kit 兼容illumina平台和MGI双平台RNA建库试剂盒

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FAQ

产品文档

已发表文献

产品应用

产品介绍

total RNA建库试剂盒，搭配mRNA抓取或者核糖体去除试剂盒，试剂盒兼容链特异以及非链特异建库，兼容Illumina测序体系以及MGI测序体系。

Hieff NGS® Ultima Dual-mode RNA Library Prep Kit是兼容Illumina和MGI双平台的total RNA测序文库构建试剂盒，包含RNA片段化试剂，反转录试剂，常规和链特异性ds-cDNA合成试剂，以及文库扩增试剂。可以衔接mRNA纯化试剂盒或rRNA去除试剂盒构建测序文库。二链合成模块配有两种Buffer，客户可根据需要进行常规建库或链特异性建库。其中链特异性二链合成Buffer中将dTTP替换为dUTP，使cDNA第二链中掺入dUTP，而本试剂盒使用的高保真DNA聚合酶无法扩增含尿嘧啶的DNA模板，实现链特异性。提供的所有试剂都经过严格的质量控制和功能验证，最大程度上保证了文库构建的稳定性和重复性。

产品特色

流程缩减：二链合成、末端修复、加A三步合为一步。
兼容链特异和非链特异文库：选用dNTP或者dUTP扩增，实现非链特异或链特异文库构建。
兼容Illumina和MGI文库：选用primer mix for illumina或primer mix for MGI，实现双平台兼容。

应用案例

产品应用：mRNA建库、lncRNA建库、m6A建库、RIP-seq、CLIP-seq、降解组、翻译组等

1、RNA片段化条件选择

通过对片段化体系进行优化，本试剂盒mRNA片段化效果更集中，效果更好。片段化条件选择如下：

94℃ 5min : 400~500bp;
94℃ 7min : 350~400bp;
94℃ 10min : 250~300bp。

2、植物样本建库数据展示

不同投入量的拟南芥样本进行RNA建库，RNA文库产量相当或者更高，基因检出数更多， Dup值更低，自连率较低。

3、不同质量RNA样本建库的数据展示

不同DV200 Human FFPE RNA的500ng投入量建库，建库产量高，数据利用率高，Mapping Rate 略高，rRNA残留率低，外显子比例高，样本质量越低，Dup值低。

*DV200 表示样本中大于200nt的RNA 片段所占的比例，对于降解严重的FFPE 样本，DV200值能够更好的反应样本的质量。

存储条件

-25~-15℃保存。

COA

已发表文献

[ 1 ]Ovarian Cancer Diagnosis and Chemoresistance Prediction Model Based on cfRNA Molecular Signature

Journal:Advanced Science|DOI:10.1002/advs.76274|IF:14.1

[ 2 ]Lipidized deferoxamine nanomedicines (DFOsomes) against iron overload

Journal:JOURNAL OF CONTROLLED RELEASE|DOI:10.1016/j.jconrel.2026.114939|IF:12.4

[ 3 ]A Versatile Self-Amplifying mRNA System Consolidates Oncolytic Virus Benefits into a Single Flexible Backbone for Cancer Therapy

Journal:MOLECULAR THERAPY|DOI:10.1016/j.ymthe.2025.12.066|IF:11.4

[ 4 ]A CRISPR/Cas13a-Based One-Step System for Rapid Detection of Emerging Viruses: Deployment during the Chikungunya Outbreak in Guangdong Province, China

Journal:ACS Sensors|DOI:10.1021/acssensors.6c00452|IF:10.9

[ 5 ]D-mannose alleviates colonic inflammation in mice: modulation of the gut microbiota-host gene axis and inhibition of PI3K/AKT/NF-κB signaling

Journal:Journal of Future Foods|DOI:10.1016/j.jfutfo.2026.04.009|IF:10.6

[ 6 ]Combination therapy based on dual-target biomimetic nano-delivery system for overcoming cisplatin resistance in hepatocellular carcinoma

Journal:JOURNAL OF NANOBIOTECHNOLOGY|DOI:10.1186/s12951-023-01840-3|IF:10.2

[ 7 ]Capture sequencing demonstrates promising public health potential for post-COVID wastewater surveillance: a comparative multi-technique and spatiotemporal analysis

Journal:ENVIRONMENT INTERNATIONAL|DOI:10.1016/j.envint.2026.110146|IF:9.7

[ 8 ]Genetic dissection of cadmium accumulation in tea plants under nonphytotoxic field conditions

Journal:Journal of Genetics and Genomics|DOI:10.1016/j.jgg.2026.05.006|IF:7.9

[ 9 ]Nuclear Receptors as Lethal Targets in the White-Backed Planthopper via RNAi and Nanocarrier Delivery

Journal:JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY|DOI:10.1021/acs.jafc.5c13729|IF:6.7

[ 10 ]Mapping and characterization of stable major QTL for heading date and spikelet number in wheat

Journal:Crop Journal|DOI:10.1016/j.cj.2026.01.003|IF:6.7

[ 11 ]Early replication fragile sites are associated with cancer-related CNVs and SNVs in human embryonic stem cells

Journal:Stem Cell Reports|DOI:10.1016/j.stemcr.2026.102968|IF:5.6

[ 12 ]Age-dependent transcriptional remodeling of the liver and gallbladder in response to fasting in mice

Journal:Scientific Reports|DOI:10.1038/s41598-026-48575-3|IF:4.9

[ 13 ]Circadian and phytohormone responses underlying red and blue light control of flowering in pepino (Solanum muricatum)

Journal:SCIENTIA HORTICULTURAE|DOI:10.1016/j.scienta.2026.114640|IF:4.6

[ 14 ]Aquaculture-mediated introgression and natural selection shape population differentiation in the blood clam Anadara granosa

Journal:AQUACULTURE|DOI:10.1016/j.aquaculture.2026.744037|IF:4.4

[ 15 ]Integrative Analysis of Q-Markers and Transcriptomics Reveals the Material Basis and Mechanism of Lonicerae Japonicae Flos in Inhibiting Ferroptosis

Journal:FASEB JOURNAL|DOI:10.1096/fj.202503711RR|IF:4.3

[ 16 ]Ferulic Acid Alleviates Chemotherapy-Induced POI by Targeting the Grp78 and Perk-eIF2α-ATF4-CHOP Pathway to Attenuate Endoplasmic Reticulum Stress

Journal:Biomedicines|DOI:10.3390/biomedicines14030714|IF:3.9

[ 17 ]Integrative transcriptomic and metabolomic analyses identify lipid-related genes and metabolites associated with intramuscular fat deposition between two cattle breeds

Journal:GENOMICS|DOI:10.1016/j.ygeno.2026.111258|IF:3.6

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