B7 homolog 3 (B7-H3) is a member of the B7 family of immune proteins that provide signals for regulating immune responses. Other family members include B7-1, B7-2, B7-H2, PD-L1 (B7-H1), and PD-L2. B7 proteins are immunoglobulin (Ig) superfamily members with extracellular Ig-V-like and Ig-C-like domains and short cytoplasmic domains. Among the family members, they share about 20-40% aa sequence identity.
产品性质
Synonyms |
B7H3; B7-H3; CD276; PSEC0249; UNQ309; PRO352; B7 homolog 3; CD276 |
Uniprot No. |
Q5ZPR3-2 |
Source |
Recombinant Biotinylated Human B7-H3 Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Leu29-Pro245. |
Molecular Weight |
The protein has a predicted MW of 26.49 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. |
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Purity |
> 95% as determined by SDS-PAGE and HPLC |
Endotoxin |
< 1.0 EU per 1μg of the protein by the LAL method. |
Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100μg/mL is recommended. Dissolve the lyophilized protein in distilled water. |
运输与保存方法
The products are shipped with ice pack and can be stored at -20℃ for 1 year.
2-7 days, 2 to 8 °C under sterile conditions after reconstitution.
3 -6 months, -20 to -80°C under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.
注意事项
1.For your safety and health, please wear lab coats and disposable gloves for operation.
2.For research use only!
产品数据
HB230117