甘氨酸和水杨酸协同促进冬虫夏草素和培美曲塞的合成，其作用机制是通过上调冬虫夏草菌中生物合成基因Cns3的表达来实现的。

Abstract

This study aimed to investigate the effects of glycine (Gly) and salicylic acid (SA) on the production of cordycepin and pentostatin, two key bioactive metabolites of Cordyceps militaris , during submerged fermentation. When applied individually at optimal concentrations, both Gly and SA significantly enhanced the yields of these metabolites, while their combined supplementation led to a further increase in accumulation. Given that cordycepin and pentostatin are synthesized via the Cns3 -dependent pathway, we hypothesized that Gly and SA may influence the expression of the Cns3 gene. RT-qPCR analysis confirmed that the combined treatment upregulated Cns3 transcription levels. These results demonstrate that Gly and SA act as effective inducers, simultaneously promoting cordycepin and pentostatin biosynthesis through Cns3 activation, and provide a practical strategy for improving the fermentation efficiency of C. militaris .