Rapid Degradation of SARS-CoV-2 Spike S Protein by A Specific Serine Protease

Jiankai Liu, Mujie Kan, Lianzhi Zhang, Yuan Yue, Shaohua Wang, Min Hong, Xinyu Hong

Journal：MOLECULES

IF：4.93

DOI：10.3390/molecules27061882

PMID：35335246

Published：2022-03-14

research field：分子生物学内质网应激信号通路代谢性疾病研究植物药治疗民族药理学

Abstract

The S protein of SARS-CoV-2 is a crucial structural and functional component for virus entry. Due to the constant mutation of the virus, there are very limited ways to prevent and control COVID-19. This experiment used a macroscopic SDS-PAGE method and proved that the S protein of wild-type SARS-CoV-2 virus, especially the S1 subunit, is very sensitive to alkaline serine protease with acidic pI (ASPNJ), NJ representsNeanthes japonica(Izuka) from which ASP is purified). ASPNJ cleaves proteins when the carbonyl group of the peptide bond is contributed by arginine or lysine. ASPNJ can degrade the S protein very quickly and effectively in vitro with relative selectivity. It can be inferred that the S, S1 and RBD of SARS-CoV-2 variants can also be easily degraded by ASPNJ. This rapid and strong degradation of the S protein by ASPNJ may become a potential new treatment strategy.Keywords:SARS-CoV-2;spike (S) protein;alkaline serine protease with an acidic pI (ASPNJ);degradation of S protein;antiviral agent

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