Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55.

 

产品性质

别名	TSG101; Tumor susceptibility gene 101 protein; ESCRT-I complex subunit TSG101
SwissProt ID	Q99816
基因ID	7251
免疫原	A synthetic peptide of human TSG101
缓冲液	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
分子量	Calculated MW:44 kDa; Observed MW: 44 kDa
克隆类型	Monoclonal Antibody
克隆号	4D9
宿主	Rabbit
物种反应	Human, Mouse, Rat
推荐应用	WB, IHC-F, IHC-P, ICC/IF
纯化方式	Affinity Purified

 

应用

稀释比 WB:1/500-1/1000; IHC:1/50-1/100; IF:1/50-1/200

 

储存条件

-25~-15℃保存

 

注意事项

1. 避免反复冻融。

2. 本产品仅作科研用途。

3. 为了您的安全和健康，请穿实验服并佩戴一次性手套操作。

 

产品数据

Western blot

Western blot analysis of TSG101 in K562, Rat Brain, C6, 3T3, HeLa lysates using TSG101 antibody.

 

Immunocytochemistry

Immunocytochemistry analysis of TSG101(green) in HT-1080 using TSG101 antibody, and DAPI(blue).

 

Immunocytochemistry

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using TSG101 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

 

Ver.CN20231102