The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

稀释比  WB:1/500-1/1000; IHC:1/50-1/100; IP: 1/20

Western blot

Western blot analysis of p27 KIP1 in 3T3, HeLa lysates using p27 Kip 1 antibody.

Immunohistochemitry

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using p27 KIP1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

-25~-15℃保存