Trop-2,also known as epithelial glycoprotein-1 antigen (EGP-1),is a protein that in humans is encoded by the TACSTD2 gene.Mutations of this gene result in gelatinous drop-like corneal dystrophy, an autosomal recessive disorder characterized by severe corneal amyloidosis leading to blindness.
产品性质
别名 |
EGP1; EGP-1; TROP2; GA733-1; gp50; T16; TACSTD2; TROP-2; M1S1; TACD2 |
Uniprot No. |
P09758 |
表达区间及表达系统 |
Recombinant Biotinylated Human TROP-2/TACSTD2 Protein is expressed from HEK293 cells with His tag and Avi tag at the C-terminal. It contains His27-Thr274. |
分子量 |
The protein has a predicted MW of 30.5 kDa. Due to glycosylation, the protein migrates to 46-55 kDa based on Tris-Bis PAGE result. |
纯度 |
> 95% as determined by SDS-PAGE and HPLC |
活性 |
ELISA Data:Immobilized Anti-TROP-2 Antibody, hFc Tag at 0.5 μg/mL (100 μL/well) on the plate. Dose response curve for Biotinylated Human TROP-2, His Tag with the EC50 of 24.1 ng/mL determined by ELISA. |
内毒素 |
< 1.0 EU per 1μg of the protein by the LAL method. |
制剂 |
Lyophilized from 0.22 μm filtered solution in PBS (pH7.4). Normally 5% trehalose is added as protectant before lyophilization. |
复溶 |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
运输与保存方法
冰袋运输。-20℃保存,一年有效期。
复溶后,-20 至 -80°C,在未开封状态下保存 3-6 个月。复溶后,2-8°C 保存 2-7 天。
建议第一次使用时分装冻存,避免反复冻融。
注意事项
1. 避免反复冻融。
2. 为了您的安全和健康,请穿实验服并戴一次性手套操作。
3. 本产品仅作科研用途!
产品数据
HB220930