CEACAM-5, also known as CEA and CD66e, belongs to the large family of CEACAM and pregnancy specific glycoproteins. CEACAM molecules are either transmembrane or GPI-linked, and are differentially expressed between species. Orthologs of human CEACAM-5 have not been described in other species. CEACAM-5, which is expressed primarily by epithelial cells, consists of an N-terminal Ig-like V-set domain followed by six Ig-like C2-set domains and a GPI anchor. CEACAM-5 is synthesized as a 180 kDa, variably glycosylated molecule of which approximately 60% is carbohydrate. CEACAM-5 functions as a calcium-independent adhesion molecule through homophilic and heterophilic interactions with CEACAM-1. CEACAM-5 is restricted to the apical face of intestinal epithelial cells in the adult but is more diffuse during embryonic development and in tumors. This is consistent with a role in the development and maintenance of epithelial architecture. CEACAM-5 is up-regulated in a wide variety of human tumors and is a commonly used cancer marker. It promotes tumor cell migration, invasion, adhesion, and metastasis. It also contributes to tumor formation by maintaining cellular proliferation in the presence of differentiation stimuli, and by blocking apoptosis following loss of ECM anchorage (anoikis).

 

产品性质

别名（Synonyms）	CEACAM-5; CD66e; CEA; Meconium antigen 100; Carcinoembryonic; CEACD66e; DKFZp781M2392
Uniprot No.	Q8N4D0
表达区间及表达系统（Source）	Recombinant Human CEACAM-5/CD66e Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Lys35-Ala685.
分子量(Molecular Weight)	The protein has a predicted MW of 74.2 kDa. Due to glycosylation, the protein migrates to 115-150 kDa based on Tris-Bis PAGE result.
外观(Physical Appearance）	Sterile Filtered White lyophilized (freeze-dried) powder.
纯度（Purity）	> 95% as determined by SDS-PAGE and HPLC
活性（Activity）	1.ELISA Data：Immobilized Human CEACAM-5 at 0.5μg/ml (100μl/well). Dose response curve for Anti-CEACAM Antibody, hFc Tag with the EC50 of 14.8ng/ml determined by ELISA. 2.SPR Data: Human CEACAM-5, His Tag captured on CM5 Chip via Anti-His Antibody can bind Anti-CEACAM5 Antibody, hFc Tag with an affinity constant of 0.12nM as determined in SPR assay (Biacore T200).
内毒素（Endotoxin）	< 1.0 EU per 1μg of the protein by the LAL method.
制剂（Formulation）	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
复溶（Reconstitution）	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

 

运输与保存方法

冰袋运输。-20℃至-80℃保存，一年有效期。

复溶后，-20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。

建议第一次使用时分装冻存，避免反复冻融。

 

注意事项

1. 避免反复冻融。

2. 为了您的安全和健康，请穿实验服并戴一次性手套操作。

3. 本产品仅作科研用途！

 

产品数据

HB220624