产品说明书
FAQ
COA
已发表文献
HGFR is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. The protein possesses tyrosine kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Within the ECD, human HGFR shares 86%-88% aa sequence identity with canine, mouse, and rat HGFR.
产品性质
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Synonyms |
MET; oncogene MET; HGF R; HGF/SF receptor; AUTS9; cMET; Met (c-Met); RCCP2; SF receptor |
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Uniprot No. |
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Source |
Recombinant Biotinylated Human HGF R/c-MET Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Glu25-Thr932. |
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Molecular Weight |
The mature form of HGF R is a heterodimer which can be cleaved into α and β chain. The protein has a predicted MW of 32.5 kDa (α chain) and 72.1 kDa (β chain). Due to glycosylation, the protein migrates to 45-50 kDa and 80-90 kDa based on Tris-Bis PAGE result. |
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Purity |
> 95% as determined by SDS-PAGE and HPLC |
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Activity |
ELISA Data: Immobilized Anti-HGF R Antibody, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human HGFR, His Tag with the EC50 of 25.7ng/ml determined by ELISA. |
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Endotoxin |
< 1.0 EU per 1μg of the protein by the LAL method. |
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Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
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Reconstitution |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
运输与保存方法
The products are shipped with ice pack and can be stored at -20℃ for 1 year.
2-7 days, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 °C under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.
注意事项
1.For your safety and health, please wear lab coats and disposable gloves for operation.
2.For research use only!
产品数据
HB221103
