产品说明书
FAQ
COA
已发表文献
OX40 Ligand (OX40L), also known as gp34, is a type II transmembrane glycoprotein belonging to the TNF superfamily. Human OX40L cDNA encodes a 183 amino acids (aa) polypeptide with an amino-terminal cytoplasmic domain (aa 1-23) and a carboxy-terminal extracellular domain (aa 51-183). It shares 46% aa sequence identity with the mouse counterpart. OX40L is expressed on the surface of activated B cells, T cells, dendritic cells and endothelial cells. Engagement of OX40 on activated T cells in situ in tumors has been shown to augment immune responses and subsequent tumor regression.
产品性质
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别名（Synonyms） |
CD134, OX40L receptor, TNFRSF4, ACT-135, Ly-70, OX40 homologue, TXGP1L, IMD16 |
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Uniprot No. |
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表达区间及表达系统（Source） |
HEK293 Cells-derived Human OX40 / TNFRSF4 / CD134 Protein, Leu29-Ala216 with hFc tag at the C-terminal |
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分子量(Molecular Weight) |
Approximately 46.8 kDa. Due to glycosylation, the protein migrates to 70-78 kDa based on Tris-Bis PAGE result. |
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外观(Physical Appearance） |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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纯度（Purity） |
> 95% as determined by SDS-PAGE |
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活性（Activity） |
ELISA Data: Immobilized Human OX40 Ligand (Trimer) , His Tag at 1 μg/mL (100 μL/well) on the plate. Dose response curve for Human OX40, hFc Tag with the EC50 of 0.27 μg/mL determined by ELISA. |
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内毒素（Endotoxin） |
< 1.0 EU per 1μg of the protein by the LAL method. |
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制剂（Formulation） |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
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复溶（Reconstitution） |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
运输与保存方法
冰袋运输。-20℃至-80℃保存，一年有效期。
复溶后，-20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。
建议第一次使用时分装冻存，避免反复冻融。
注意事项
1. 避免反复冻融。
2. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
3. 本产品仅作科研用途！
产品数据
HB220629
