产品说明书
FAQ
COA
已发表文献
TIGIT, also called Vstm3, Vsig9 and WUCAM is a 30-34 kDa type I transmembrane protein that is a member of the CD28 family within the Ig superfamily of proteins. Within the ECD, human TIGIT shares only 65% and 67% aa sequence identity with mouse and rat TIGIT. It binds to CD155/PVR/Necl-5 and Nectin-2/CD112/PVRL2 that appear on dendritic cells (DC) and endothelium. Binding of TIGIT by DC induces IL-10 release and inhibits IL-12 production. Ligation of TIGIT on T cells downregulates TCR-mediated activation and subsequent proliferation, while in NK cell, TIGIT ligation blocks NK cell cytotoxicity. In particular, TIGIT binding to CD155 can antagonize the effects of DNAM-1. Soluble TIGIT is able to compete with DNAM-1 for CD155 binding and attenuates T cell responses, while mice lacking TIGIT show increased T cell responses and susceptibility to autoimmune challenges.
产品性质
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别名（Synonyms） |
TIGIT, VSIG9, VSTM3, WUCAM |
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Uniprot No. |
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表达区间及表达系统（Source） |
HEK293 Cells-derived Human TIGIT Protein, Met22-Pro141 with His tag at the C-terminal |
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分子量(Molecular Weight) |
Approximately 14 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Tris-Bis PAGE result. |
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外观(Physical Appearance） |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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纯度（Purity） |
> 95% as determined by SDS-PAGE and HPLC |
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活性（Activity） |
1. ELISA Data: Immobilized Human TIGIT, His Tag at 0.5 μg/mL (100 μL/well) on the plate. Dose response curve for Anti-TIGIT Antibody, hFc Tag with the EC50 of 18 ng/mL determined by ELISA. 2. SPR Data: Human CD155, hFc Tag captured on Protein A chip, can bind Human TIGIT, His Tag with an affinity constant of 0.17 μM as determined in a SPR assay (Biacore T200). |
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内毒素（Endotoxin） |
< 1.0 EU per 1μg of the protein by the LAL method. |
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制剂（Formulation） |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
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复溶（Reconstitution） |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
运输与保存方法
冰袋运输。-20℃至-80℃保存，一年有效期。
复溶后，-20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。
建议第一次使用时分装冻存，避免反复冻融。
注意事项
1. 避免反复冻融。
2. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
3. 本产品仅作科研用途！
产品数据
HB220630
