推荐应用
Interleukin-17B (IL-17B), is an approximately 20 kDa glycosylated cytokine that plays a role in inflammation and bone growth. The six IL-17 cytokines (IL-17A-F) are encoded by separate genes but adopt a conserved cystine knot fold. Mature human IL-17B shares 90% and 91% amino acid sequence identity with mouse and rat IL-17B, respectively. IL-17B binds to IL-17 RB and induces the production of inflammatory cytokines and the infiltration of inflammatory immune cells (3, 4). IL-17B is expressed in the central nervous system, testis, ovary, stomach, pancreas, and small intestine
高纯度、高活性、低内毒素、高批间一致性
复溶方法
1. 在打开盖子之前,先使用约 13000 rpm 的转速进行离心,20-30 s,将附着在管盖或管壁上的冻干粉收集到管底,以避免损失。
2. 向冻干粉中加入提供的复溶缓冲液,用移液枪轻吹混匀。重悬至浓度不低于100 μg/mL。勿涡旋剧烈振荡。
3. 复溶后的重组蛋白在室温放置数分钟,以保证蛋白能够充分溶解。
4. 短期实验 (不超过7天),用复溶缓冲液直接溶解的重组蛋白液体可在 2-8°C 保存 1 周。不建议复溶后直接冻存。
5. 如需长期保存,可使用包含一定浓度载体蛋白(0.1% BSA,5% HSA,10% FBS,或 5% 海藻糖)的溶液或培养基复溶产品。无血清实验需求时,可选择 5% 海藻糖溶液作为载体。
6. 以10 μg为例,先加20 μL我们赠送的复溶液,移液器吹打充分溶解。
7. 再加 80 μL 含载体蛋白(0.1% BSA, 5% HSA, 10% FBS, 或 5% 海藻糖中任一种)的缓冲液/培养基,移液器吹打混匀;最终浓度不低于100 μg/mL。
8. 分装体积不少于 20 μL/管。
9. 分装后冻存于 -20ºC~-80ºC,可保存 3~6 个月。
SDS-PAGE

The purity is greater than 95% as determined by SDS-PAGE.
Bioactivity

Measured by its ability to induce IL-8 secretion by HepG2 cells. The ED50 for this effect is 1.169 μg/mL.
Corresponding to a specific activity is 855.432 U/mg.
-25 ~ -15℃保存,收到货之后有效期1年。
复溶后,无菌条件下,2~8℃保存,7天有效期。复溶后, 无菌条件下,-85 ~ -65℃保存,3个月有效期。





