产品简介

 

Interleukin 7 (IL-7) is a cytokine involved in the regulation of immune cell development and survival, including T and B lymphocytes. It is produced by stromal cells, such as fibroblasts and bone marrow stromal cells. Dysregulation of IL-7 signaling has been implicated in autoimmune diseases and cancer.

 

性能参数

Synonyms	Interleukin 7;IL7; IL-7; IL-7interleukin-7; interleukin 7; Lymphopoietin-1; PBGF
Uniprot No.	P13232
Source	Recombinant Human IL-7 Protein is expressed from E.coli . It contains Asp 26-His 177.
Tag	No Tag.
Molecular Weight	Approximately 17.4 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids.
Purity	> 95% as determined by SDS-PAGE and HPLC.
Endotoxin	<1.0 EU per 1μg of the protein by the LAL method.
Formulation	Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, 150 mM NaCl, pH 7.4.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Reconstitution	Centrifuge tubes before opening. Dissolve lyophilized protein with sterile  PBS to ensure the concentration is greater than 100 ug/mL.

 

 

储存条件

 

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~8 °C under sterile conditions after reconstitution.

3 months, -25~-15℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

 

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

 

产品数据

Bioactivity-Cell based assay 

Figure 2. The ED₅₀ as determined by a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL) is less than 1 ng/mL, corresponding to a specific activity of >1 10⁶ IU/mg.

 

 

Ver. CN20241021