Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family. Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf 1. Apaf-1 mediated activation of caspase-9 involves instrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit.
推荐稀释比 WB:1/500-1/1000; IHC:P:1/100-1/200; ICC/IF:1/200-1/500; IP:1/50-1/100
Western blot analysis of Caspase9 in HeLa lysates using Caspase 9 antibody.
Immunohistochemistry analysis of paraffin-embedded Human liver using Caspase9 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Immunofluorescence analysis of Caspase 9 in HeLa treated with staurosporine using Caspase9 antibody.
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.