Mesothelin, also known as CAK1 and ERC, is derived from a 70 kDa precursor that also includes Megakaryocyte Potentiating Factor (MPF). The 70 kDa precursor is expressed on the cell surface where it is cleaved at a dibasic proteolytic site to release the 32 kDa glycosylated MPF. MPF is a cytokine that potentiates IL-3 induced megakaryocyte colony formation. The term Mesothelin refers to the 40 kDa glycosylated protein which remains attached to the cell surface via a GPI linkage. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.

高纯度、高活性、低内毒素、高批间一致性

                                  流式分析

2e5 of transient transfected anti- MSLN ScFv CAR-293 cells were stained with 0.1ug PE-Labeled MSLN Fc&Avi Tag Protein, Human and unlable respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). PE signal was used to evaluate the binding activity.

2e5 of transient transfected anti- MSLN ScFv CAR-293 cells were stained with isotype and Whitlow/218 Linker-Alexa Fluor® 488 (Fig. D and E). Alexa Fluor® 488 signal was used to evaluate the binding activity.

-85 ~ -65℃保存，收到货之后有效期6个月。
避免反复冻融，打开小瓶前先离心。建议等分分装为一次实验使用量，分装后，-80℃冻存。