产品说明书
FAQ
COA
已发表文献
CD19 protein is a 95 kDa transmembrane glycoprotein that plays a central role in B cell activation and humoral immune responses. Within the ECD, human CD19 shares 57% amino acid sequence identity with mouse and rat CD19. It is required for the responsiveness of mature B cell to antigen stimulation, germinal center development, and antibody affinity maturation. CD19 polymorphisms and up-regulation can lead to the development of autoimmunity by promoting autoantibody production. CD19 has emerged as promising therapeutic target for hematologic cancers and solid tumors. Immunotherapy using a chimeric antigen receptor (CAR) targeting CD19 has proven effective in many different cancers.
产品性质
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别名（Synonyms） |
CD19, B4, CVID3, Leu-12, MGC12802 |
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Uniprot No. |
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表达区间及表达系统（Source） |
Recombinant Human CD19 Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Pro20-Lys291. |
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分子量(Molecular Weight) |
Approximately 34.7 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result. |
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外观(Physical Appearance） |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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纯度（Purity） |
> 95% as determined by SDS-PAGE |
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活性（Activity） |
ELISA Data: Immobilized Human CD19 Protein at 5 μg/mL can bind different Anti-CD19 Antibodies with high affinity. |
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内毒素（Endotoxin） |
< 1.0 EU per 1μg of the protein by the LAL method. |
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制剂（Formulation） |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
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复溶（Reconstitution） |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
运输与保存方法
冰袋运输。-20℃至-80℃保存，一年有效期。
复溶后，-20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。
建议第一次使用时分装冻存，避免反复冻融。
注意事项
1. 避免反复冻融。
2. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
3. 本产品仅作科研用途！
产品数据
Tris-Bis PAGE
HB220701
