生殖系特异性线粒体融合调控因子Pld6在生殖干细胞维持和分化中的关键作用
A Germline-Specific Regulator of Mitochondrial Fusion is Required for Maintenance and Differentiation of Germline Stem and Progenitor Cells
Ru Zhang, Yi-Xuan Tu, Ding Ye, Zhenglong Gu, Zhen-Xia Chen, Yonghua Sun
Journal：Advanced Science
IF：17.52
DOI：10.1002/advs.202203631
PMID：36257818
Published：2022-10-18
research field：分子生物学细胞生物学代谢发育生物学表观遗传学
Abstract
Maintenance and differentiation of germline stem and progenitor cells (GSPCs) is important for sexual reproduction. Here, the authors identify zebrafish pld6 as a novel germline-specific gene by cross-analyzing different RNA sequencing results, and find that pld6 knockout mutants develop exclusively into infertile males. In pld6 mutants, GSPCs fail to differentiate and undergo apoptosis, leading to masculinization and infertility. Mitochondrial fusion in pld6 -depleted GSPCs is severely impaired, and the mutants exhibit defects in piRNA biogenesis and transposon suppression. Overall, this work uncovers zebrafish Pld6 as a novel germline-specific regulator of mitochondrial fusion, and highlights its essential role in the maintenance and differentiation of GSPCs as well as gonadal development and gametogenesis.
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