Construction of a peacock immortalized fibroblast cell line for avian virus production
Jie Wang, Xiangyu Yu, Shurui Zhao, Nian Zhang, Zhenyu Lin, Zhaofei Wang, Jingjiao Ma, Yaxian Yan, Jianhe Sun, Yuqiang Cheng
Journal：POULTRY SCIENCE
IF：4.01
DOI：10.1016/j.psj.2022.102147
PMID：36191515
Published：2022-08-27
research field：分子生物学免疫学传染病学微生物学
Abstract
ABSTRACT The mammalian-derived MDCK cells are the most widely used for avian virus vaccine production at present. The use of heterologous cell systems for avian virus preparation may cause security risks. An avian cell line is available for avian virus vaccines urgently needed. In this study, a peacock immortalized fibroblast cell line that is suitable for avian virus vaccine production was generated. The primary peacock fibroblast cells were prepared, and the immortal cells PEF-1 were obtained by transferring hTERT into the primary cells and screening with G418. The PEF-1 has high cell viability and expresses exogenous TERT protein. More importantly, the virus replication ability was stronger in PEF-1 than in MDCK cells as evaluated by virus fluorescence and TCID 50 , after being infected with NDV-GFP, VSV-GFP, and AIV. In conclusion, the peacock immortalized PEF cells are expected to be used for the production of peacock and other avian virus vaccines.
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