长链非编码RNA LINC02323通过miR-1343-3p/TGF-β受体1促进卵巢癌生长迁移

Abstract

Background This study was aimed at investigating the effects of long noncoding RNA (lncRNA) LINC02323 in ovarian cancer and its possible mechanism. Methods Microarray analysis and QPCR were utilized to identify lncRNA LINC02323 expression in patients with ovarian cancer. MTT assay was used for analysis of ovarian cancer cell proliferation. Western blot was utilized to investigate its possible mechanism. Results In patients with ovarian cancer, lncRNA LINC02323 expression was up-regulated and miR-1343-3p expression was down-regulated. Over-expression of lncRNA LINC02323 promoted cell growth and reduced LDH activity levels in vitro model by suppression of miR-1343-3p expression. Down-regulation of lncRNA LINC02323 reduced cell growth and increased LDH activity levels in vitro model by induction of miR-1343-3p expression. Over-expression of miR-1343-3p reduced cell growth and reduced LDH activity levels in vitro model by suppression of TGF-β receptor. Down-regulation of miR-1343-3p promoted cell growth and reduced LDH activity levels in vitro model by induced of TGF-β receptor. Conclusion Our findings show that Novel long noncoding RNA LINC02323 promotes cell growth of ovarian cancer via TGF-β receptor 1 by miR-1343-3p.