基于聚邻苯二胺-对苯二酚分子印迹传感器的beta-淀粉样蛋白42检测方法

Abstract

A sensitive and selective molecularly imprinted polymer (MIP) sensor was developed for the determination of amyloid-β (1–42) (Aβ 42 ). The glassy carbon electrode (GCE) was successively modified with electrochemical reduction graphene oxide (ERG) and poly(thionine-methylene blue) (PTH-MB). The MIPs were synthesized by electropolymerization with Aβ 42 as a template and o-phenylenediamine (o–PD) and hydroquinone (HQ) as functional monomers. Cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), chronoamperometry (CC), and differential pulse voltammetry (DPV) were used to study the preparation process of the MIP sensor. The preparation conditions of the sensor were investigated in detail. In optimal experimental conditions, the response current of the sensor was linear in the range of 0.12–10 μg mL −1 with a detection limit of 0.018 ng mL −1 . The MIP-based sensor successfully detected Aβ 42 in commercial fetal bovine serum (cFBS) and artificial cerebrospinal fluid (aCSF).