Chicken interferon regulatory factor 1 (IRF1) involved in antiviral innate immunity via regulating IFN-β production
Yunxia Liu, Yuqiang Cheng, Wenya Shan, Jingjiao Ma, Hengan Wang, Jianhe Sun, Yaxian Yan
Journal：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
IF：2.91
DOI：10.1016/j.dci.2018.07.003
PMID：29981306
Published：2018-07-05
research field：分子生物学免疫学病毒学
Abstract
Interferon regulatory factors (IRFs) is an important family for IFN expression regulating while viral infection . IRF1 , IRF3 , and IRF7 are the primary regulators that trigger type I IFN response in mammals. However, IRF3, which has been identified as the most critical regulator in mammals, is absent in chickens, and it is unknown whether IRF1 is involved in type I IFN signaling pathways in IRF3-deficient chicken cells. Here, we identified chicken IRF1 (chIRF1) as a critical IFN-β mediator in response to viral infection. Overexpression of chIRF1 activated IFN-β intensively and suppressed AIV and NDV viral replication. Moreover, the mRNA levels of IFN-β and ISGs increased during chIRF1 overexpression. In addition, deletion mutant analysis revealed that the first four domains of chIRF1 are indispensable for IFN-β induction. Together, our studies demonstrate that chIRF1 is an important regulator of IFN-β and is involved in chicken antiviral innate immunity .
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