RecombinantHuman/Mouse Wnt-5a Protein, His Tag 重组人/小鼠 Wnt5a蛋白

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产品说明书

FAQ

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产品信息

 

产品名称

产品编号

规格

Recombinant Human/Mouse Wnt-5a Protein, His Tag

    重组人/小鼠 Wnt5a蛋白

92282ES10

10 μg

92282ES60

100 μg

92282ES76

500 μg

 

性能参数

 

别名(Synonyms)

Wingless-type MMTV Integration Site Family, Member 5a; wingless-type MMTV integration site family, member 5A; Wnt5a

表达系统(Source)

HEK293 Cells

表达序列(Sequence)

Gln38-Lys380

Accession

P41221

分子量大小(Molecular Weight)

The protein has a predicted MW of 40kDa. The protein migrates as 60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation

纯度(Purity)

> 90% by SDS-PAGE.

内毒素(Endotoxin)

< 1.0 EU per 1μg of the protein by the LAL method.

生物活性(Biological Activity)

Measured by its ability to inhibit Wnt-3a-induced a TCF/LEF luciferase reporter

expression by 293F cells. The ED50 for this effect is 2-10µg/mL. Optimal concentrations

should be determined by each laboratory for each application

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 5% trehalose and 0.01 % Tween 80 were added as protectant before lyophilization

标签(Tag)

His tag at the C-terminal

外观(Physical Appearance)

冻干粉

Reconstitution

Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

 

储存条件

 

-85~-65℃保存,收到货之后有效期1年。

避免反复冻融,打开小瓶前先离心。建议等分分装为一次实验使用量,分装后,-80℃冻存。

 

复溶方法

 

开盖前,先离心。在含有至少0.1%人或牛血清白蛋白的无菌PBS中复溶,复溶到100μg/mL的浓度。

 

注意事项

  1. 为了您的安全和健康,请穿实验服并戴一次性手套操作。
  2. 本产品仅作科研用途。

 

Ver.CN20240909

400-6111-883