产品信息
产品名称 |
产品编号 |
规格 |
Recombinant Human/Mouse Wnt-5a Protein, His Tag 重组人/小鼠 Wnt5a蛋白 |
92282ES10 |
10 μg |
92282ES60 |
100 μg |
|
92282ES76 |
500 μg |
性能参数
别名(Synonyms) |
Wingless-type MMTV Integration Site Family, Member 5a; wingless-type MMTV integration site family, member 5A; Wnt5a |
表达系统(Source) |
HEK293 Cells |
表达序列(Sequence) |
Gln38-Lys380 |
Accession |
P41221 |
分子量大小(Molecular Weight) |
The protein has a predicted MW of 40kDa. The protein migrates as 60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation |
纯度(Purity) |
> 90% by SDS-PAGE. |
内毒素(Endotoxin) |
< 1.0 EU per 1μg of the protein by the LAL method. |
生物活性(Biological Activity) |
Measured by its ability to inhibit Wnt-3a-induced a TCF/LEF luciferase reporter expression by 293F cells. The ED50 for this effect is 2-10µg/mL. Optimal concentrations should be determined by each laboratory for each application |
制剂(Formulation) |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 5% trehalose and 0.01 % Tween 80 were added as protectant before lyophilization |
标签(Tag) |
His tag at the C-terminal |
外观(Physical Appearance) |
冻干粉 |
Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
储存条件
-85~-65℃保存,收到货之后有效期1年。
避免反复冻融,打开小瓶前先离心。建议等分分装为一次实验使用量,分装后,-80℃冻存。
复溶方法
开盖前,先离心。在含有至少0.1%人或牛血清白蛋白的无菌PBS中复溶,复溶到100μg/mL的浓度。
注意事项
Ver.CN20240909