产品说明书
FAQ
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已发表文献
产品描述
DAPI Fluoromount-G™是一种含有蓝色细胞核荧光探针-DAPI（Em=455 nm）的水溶性封片剂，用来对最后一步染色是在水溶液体系中完成的玻片样本进行封片，可以显著减缓各种常见荧光染料的荧光淬灭现象。本封片剂具有半永久性封固性，可用作长期保存玻片样本的制作。适用于细胞制备物和组织切片的封片，兼容于免疫荧光，免疫细胞化学，免疫组化等实验。
本品已加DAPI，可同时完成核染和封片，使得实验操作更简便。封片剂干燥后形成一层牢固的涂层，光学透明，便于后续荧光观察和拍照。
运输和保存方法
室温运输。室温避光保存，一年有效。
使用方法
1） 根据实验室已有的体系制备细胞离心涂片；
2） 固定和再水化细胞涂片；
3） 从染色最后一步的水溶性缓冲液中取出一片玻片，直接滴加一滴 DAPI Fluoromount-G™至细胞涂片，盖上盖玻片，用无纺纱布轻轻按压盖玻片以去除多余分封片剂和封固盖玻片。
4） 室温下避光静置5 min，使得封片剂晾干后再进行普通荧光显微镜或者共聚焦显微检测。5） 若玻片样本需长久保存，可使用指甲油或者中性树胶（货号：36313ES60）对盖玻片四周进行密封。
注意事项
1）本品含有0.1%的叠氮钠作为防腐剂，吞食和吸入有毒，操作时请做好防护。
2）为了您的安全和健康，请穿实验服并戴一次性手套操作。
3）本产品仅作科研用途！
Q：该产品需要低温贮藏吗？
A：室温避光保存即可。
Q：使用该产品后多久可进行显微检测？
A：一般室温下避光静置 5 min，使得封片剂晾干后，再进行普通荧光显微镜或者共聚焦显微检测。
Q：该产品可使玻片长久保存吗？
A：不可。若玻片样本需长久保存，可使用指甲油或者中性树胶对盖玻片四周进行密封。
Q：该产品可用于免疫荧光、免疫细胞化学、免疫组化实验吗？
A：可以。
Q：该产品可用于活细胞吗？
A：不推荐，对于染色活细胞膜染料和线粒体染料的抗淬灭效果不佳，建议抗荧光淬灭封片剂用于固定透化的细胞或者组织。
Q：封片后片子一般如何保存？
A：4度保存。
Q：封片剂可以让荧光保持多久呢? DAPI一般可以保存多久？
A：取决于染料的本身，有的染料本身就特别容易淬灭，也是维持不了很久的，2-3天左右;对于那种相对稳定的染料，可以维持1周以上；DAPI比较稳定，可保存一周左右。
Q：此产品中DAPI试剂的浓度是多少？
A：1.5 ug/mL.
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