产品说明书
FAQ
COA
已发表文献
产品简介
本品是由辣根过氧化酶（HRP）标记的山羊抗兔IgG（H+L），使用抗原偶联的琼脂糖微珠从山羊抗血清内亲和色谱纯化所得。免疫电泳和/或ELISA法检测显示本品特异性结合完整的兔IgG分子，也会与其他兔免疫球蛋白的轻链结合。可能会与其他物种免疫球蛋白发生交叉反应，但不会识别非免疫球蛋白类的血清蛋白。
本品适合做单标实验。要做多标（Multiple-labeling）实验，建议使用经过与相近物种血清蛋白或者免疫球蛋白预先经过亲和吸附处理的二抗。
翌圣为您提供Western Blot实验整体解决方案，相关产品选购请参考：WB实验系列产品-选购指南
产品应用
建议稀释浓度：
IHC/ICC：1:500-1:5000；
ELISA/WB(显色法)：1:5000-1:100000；
ELISA/WB(化学发光法)：1:10000-1:200000；
产品信息
|
抗体浓度（Antibody Concentration） |
100 μL（0.4 mg/mL） |
|
缓冲液（Buffer） |
0.005M 磷酸钠，0.125M 氯化钠，pH7.6 |
|
稳定剂（Stabilizer） |
7.5 mg/mL BSA（无IgG，蛋白酶)， 50%甘油 |
|
防腐剂（Preservative） |
None（叠氮化钠会抑制HRP活性） |
|
原料来源（Source of Material） |
Jackson Immunoresearch 111-035-003 |
储存条件
-25 ~ -15℃分装保存，尽量避免反复冻融。有效期1年。
注意事项
1）本品不能保存于叠氮化钠中其会抑制辣根过氧化酶（HRP）活性；
2）为了您的安全和健康，请穿实验服并戴一次性手套操作。
3）本产品仅作科研用途！
暂无内容
[1] Wang Z, Lu Z, Lin S, et al. Leucine-tRNA-synthase-2-expressing B cells contribute to colorectal cancer immunoevasion. Immunity. 2022;55(6):1067-1081.e8. doi:10.1016/j.immuni.2022.04.017(IF:43.474)
[2] Xia B, Shen X, He Y, et al. SARS-CoV-2 envelope protein causes acute respiratory distress syndrome (ARDS)-like pathological damages and constitutes an antiviral target. Cell Res. 2021;31(8):847-860. doi:10.1038/s41422-021-00519-4(IF:25.617)
[3] Yang WQ, Xiong QP, Ge JY, et al. THUMPD3-TRMT112 is a m2G methyltransferase working on a broad range of tRNA substrates. Nucleic Acids Res. 2021;49(20):11900-11919. doi:10.1093/nar/gkab927(IF:16.971)
[4] Qian Y, Liu Q, Li P, et al. Highly Tumor-Specific and Long-Acting Iodine-131 Microbeads for Enhanced Treatment of Hepatocellular Carcinoma with Low-Dose Radio-Chemoembolization. ACS Nano. 2021;15(2):2933-2946. doi:10.1021/acsnano.0c09122(IF:15.881)
[5] Wang K, Jiang L, Hu A, et al. Vertebral-specific activation of the CX3CL1/ICAM-1 signaling network mediates non-small-cell lung cancer spinal metastasis by engaging tumor cell-vertebral bone marrow endothelial cell interactions. Theranostics. 2021;11(10):4770-4789. Published 2021 Mar 4. doi:10.7150/thno.54235(IF:11.556)
[6] Han L, Lai H, Yang Y, et al. A 5'-tRNA halve, tiRNA-Gly promotes cell proliferation and migration via binding to RBM17 and inducing alternative splicing in papillary thyroid cancer. J Exp Clin Cancer Res. 2021;40(1):222. Published 2021 Jul 5. doi:10.1186/s13046-021-02024-3(IF:11.161)
[7] Gao JP, Xu P, Wang M, et al. Nod factor receptor complex phosphorylates GmGEF2 to stimulate ROP signaling during nodulation. Curr Biol. 2021;31(16):3538-3550.e5. doi:10.1016/j.cub.2021.06.011(IF:10.834)
[8] Wang T, Hu J, Luo H, et al. Photosensitizer and Autophagy Promoter Coloaded ROS-Responsive Dendrimer-Assembled Carrier for Synergistic Enhancement of Tumor Growth Suppression. Small. 2018;14(38):e1802337. doi:10.1002/smll.201802337(IF:9.598)
[9] Wu X, Qiao S, Wang W, et al. Melatonin prevents peri‑implantitis via suppression of TLR4/NF-κB. Acta Biomater. 2021;134:325-336. doi:10.1016/j.actbio.2021.07.017(IF:8.947)
[10] Fang DD, Tang Q, Kong Y, et al. MDM2 inhibitor APG-115 synergizes with PD-1 blockade through enhancing antitumor immunity in the tumor microenvironment. J Immunother Cancer. 2019;7(1):327. Published 2019 Nov 28. doi:10.1186/s40425-019-0750-6(IF:8.728)
[11] Song Y, Guo Y, Li X, et al. RBM39 Alters Phosphorylation of c-Jun and Binds to Viral RNA to Promote PRRSV Proliferation. Front Immunol. 2021;12:664417. Published 2021 May 17. doi:10.3389/fimmu.2021.664417(IF:7.561)
[12] Wang C, Qu L, Li S, et al. Discovery of First-in-Class Dual PARP and EZH2 Inhibitors for Triple-Negative Breast Cancer with Wild-Type BRCA. J Med Chem. 2021;64(17):12630-12650. doi:10.1021/acs.jmedchem.1c00567(IF:7.446)
[13] Song M, Wang Y, Chen Z, et al. Human CYP enzyme-activated genotoxicity of 2,2',4,4'-tetrabromobiphenyl ether in mammalian cells. Chemosphere. 2022;291(Pt 1):132784. doi:10.1016/j.chemosphere.2021.132784(IF:7.086)
[14] Liu S, Xu DS, Li M, et al. Icariin attenuates endothelial-mesenchymal transition via H19/miR-148b-3p/ELF5 in ox-LDL-stimulated HUVECs. Mol Ther Nucleic Acids. 2020;23:464-475. Published 2020 Dec 3. doi:10.1016/j.omtn.2020.11.021(IF:7.032)
[15] Wang C, Zhang H, Fu J, et al. Bartonella type IV secretion effector BepC induces stress fiber formation through activation of GEF-H1. PLoS Pathog. 2021;17(1):e1009065. Published 2021 Jan 28. doi:10.1371/journal.ppat.1009065(IF:6.823)
[16] Liu W, Guan Y, Qiao S, et al. Antiaging Effects of Vicatia thibetica de Boiss Root Extract on Caenorhabditis elegans and Doxorubicin-Induced Premature Aging in Adult Mice. Oxid Med Cell Longev. 2021;2021:9942090. Published 2021 Aug 6. doi:10.1155/2021/9942090(IF:6.543)
[17] Qu L, Li S, Ji L, et al. Discovery of PT-65 as a highly potent and selective Proteolysis-targeting chimera degrader of GSK3 for treating Alzheimer's disease. Eur J Med Chem. 2021;226:113889. doi:10.1016/j.ejmech.2021.113889(IF:6.514)
[18] Lao Y, Wang Y, Chen J, et al. Synthesis and biological evaluation of 1,2,4-triazole derivatives as potential Nrf2 activators for the treatment of cerebral ischemic injury. Eur J Med Chem. 2022;236:114315. doi:10.1016/j.ejmech.2022.114315(IF:6.514)
[19] Wang C, Chen X, Liu X, et al. Discovery of precision targeting EZH2 degraders for triple-negative breast cancer. Eur J Med Chem. 2022;238:114462. doi:10.1016/j.ejmech.2022.114462(IF:6.514)
[20] Qu L, Ji L, Wang C, et al. Synthesis and evaluation of multi-target-directed ligands with BACE-1 inhibitory and Nrf2 agonist activities as potential agents against Alzheimer's disease. Eur J Med Chem. 2021;219:113441. doi:10.1016/j.ejmech.2021.113441(IF:6.514)
[21] Bu S, Wang Q, Sun J, Li X, Gu T, Lai D. Melatonin suppresses chronic restraint stress-mediated metastasis of epithelial ovarian cancer via NE/AKT/β-catenin/SLUG axis [published correction appears in Cell Death Dis. 2020 Sep 8;11(9):726]. Cell Death Dis. 2020;11(8):644. Published 2020 Aug 18. doi:10.1038/s41419-020-02906-y(IF:6.304)
[22] Ning FL, Tao J, Li DD, et al. Activating BK channels ameliorates vascular smooth muscle calcification through Akt signaling. Acta Pharmacol Sin. 2022;43(3):624-633. doi:10.1038/s41401-021-00704-6(IF:6.150)
[23] Wang C, Zhang J, Song S, et al. Imaging epileptic foci in mouse models via a low-density lipoprotein receptor-related protein-1 targeting strategy. EBioMedicine. 2021;63:103156. doi:10.1016/j.ebiom.2020.103156(IF:5.736)
[24] Xu R, Lai C, Yang F, et al. Preliminary Characterization of Two Small Insulinase-Like Proteases in Cryptosporidium parvum. Front Microbiol. 2021;12:651512. Published 2021 May 21. doi:10.3389/fmicb.2021.651512(IF:5.640)
[25] Su J, Jin C, Wu H, et al. Differential Expression of Three Cryptosporidium Species-Specific MEDLE Proteins. Front Microbiol. 2019;10:1177. Published 2019 May 29. doi:10.3389/fmicb.2019.01177(IF:5.640)
[26] Zhang S, Wang Y, Wu H, et al. Characterization of a Species-Specific Insulinase-Like Protease in Cryptosporidium parvum. Front Microbiol. 2019;10:354. Published 2019 Mar 6. doi:10.3389/fmicb.2019.00354(IF:5.640)
[27] Yang R, Li D, Yi S, Wang M. Evolutionarily conserved odorant-binding proteins participate in establishing tritrophic interactions. iScience. 2022;25(7):104664. Published 2022 Jun 23. doi:10.1016/j.isci.2022.104664(IF:5.458)
[28] Fang DD, Tang Q, Kong Y, et al. MDM2 inhibitor APG-115 exerts potent antitumor activity and synergizes with standard-of-care agents in preclinical acute myeloid leukemia models. Cell Death Discov. 2021;7(1):90. Published 2021 May 3. doi:10.1038/s41420-021-00465-5(IF:5.241)
[29] Ma Y, Zheng K, Pang Y, et al. Anti-hypertrophic effect of synovium-derived stromal cells on costal chondrocytes promotes cartilage repairs. J Orthop Translat. 2021;32:59-68. Published 2021 Jun 2. doi:10.1016/j.jot.2021.05.002(IF:5.191)
[30] Wu X, Jin S, Yang Y, et al. Altered expression of ferroptosis markers and iron metabolism reveals a potential role of ferroptosis in vitiligo. Pigment Cell Melanoma Res. 2022;35(3):328-341. doi:10.1111/pcmr.13032(IF:4.693)
[31] Li Z, Liu S, Fu T, Peng Y, Zhang J. Microtubule destabilization caused by silicate via HDAC6 activation contributes to autophagic dysfunction in bone mesenchymal stem cells. Stem Cell Res Ther. 2019;10(1):351. Published 2019 Nov 27. doi:10.1186/s13287-019-1441-4(IF:4.627)
[32] Shi X, Ding K, Zhao Q, et al. Suppression of CPSF6 Enhances Apoptosis Through Alternative Polyadenylation-Mediated Shortening of the VHL 3'UTR in Gastric Cancer Cells. Front Genet. 2021;12:707644. Published 2021 Sep 14. doi:10.3389/fgene.2021.707644(IF:4.599)
[33] Chen Z, Li Z, Jiang C, Jiang X, Zhang J. MiR-92b-3p promotes neurite growth and functional recovery via the PTEN/AKT pathway in acute spinal cord injury. J Cell Physiol. 2019;234(12):23043-23052. doi:10.1002/jcp.28864(IF:4.522)
[34] Yao H, Xu K, Zhou J, Zhou L, Wei S. A Tumor Microenvironment Destroyer for Efficient Cancer Suppression. ACS Biomater Sci Eng. 2020;6(1):450-462. doi:10.1021/acsbiomaterials.9b01544(IF:4.511)
[35] Sun X, Zhang J, Nie Q. Inferring latent temporal progression and regulatory networks from cross-sectional transcriptomic data of cancer samples. PLoS Comput Biol. 2021;17(3):e1008379. Published 2021 Mar 5. doi:10.1371/journal.pcbi.1008379(IF:4.475)
[36] Yin L, Zhang L, Luo L, et al. Berbamine reduces body weight via suppression of small GTPase Rab8a activity and activation of paraventricular hypothalamic neurons in obese mice. Eur J Pharmacol. 2022;916:174679. doi:10.1016/j.ejphar.2021.174679(IF:4.432)
[37] Fang DD, Zhu H, Tang Q, et al. FLT3 inhibition by olverembatinib (HQP1351) downregulates MCL-1 and synergizes with BCL-2 inhibitor lisaftoclax (APG-2575) in preclinical models of FLT3-ITD mutant acute myeloid leukemia. Transl Oncol. 2022;15(1):101244. doi:10.1016/j.tranon.2021.101244(IF:4.243)
[38] Li D, Qiu X, Yang J, Liu T, Luo Y, Lu Y. Generation of Human Lens Epithelial-Like Cells From Patient-Specific Induced Pluripotent Stem Cells. J Cell Physiol. 2016;231(12):2555-2562. doi:10.1002/jcp.25374(IF:4.155)
[39] Su J, Shen Y, Li N, et al. Comparative Characterization of CpCDPK1 and CpCDPK9, Two Potential Drug Targets Against Cryptosporidiosis. Microorganisms. 2022;10(2):333. Published 2022 Feb 1. doi:10.3390/microorganisms10020333(IF:4.128)
[40] Guo Y, Huo J, Wu D, et al. Simvastatin inhibits the adipogenesis of bone marrow‑derived mesenchymal stem cells through the downregulation of chemerin/CMKLR1 signaling. Int J Mol Med. 2020;46(2):751-761. doi:10.3892/ijmm.2020.4606(IF:4.101)
[41] Yang B, Li Y, Ma Y, et al. Selenium attenuates ischemia/reperfusion injury‑induced damage to the blood‑brain barrier in hyperglycemia through PI3K/AKT/mTOR pathway‑mediated autophagy inhibition. Int J Mol Med. 2021;48(3):178. doi:10.3892/ijmm.2021.5011(IF:4.101)
[42] Liu S, Xu DS, Ma JL, Huang P, Wu D, Ren LQ. LncRNA H19 Mitigates Oxidized Low-Density Lipoprotein Induced Pyroptosis via Caspase-1 in Raw 264.7 Cells. Inflammation. 2021;44(6):2407-2418. doi:10.1007/s10753-021-01511-1(IF:4.092)
[43] Zhang H, Guo F, Qi P, et al. OsHDA710-Mediated Histone Deacetylation Regulates Callus Formation of Rice Mature Embryo. Plant Cell Physiol. 2020;61(9):1646-1660. doi:10.1093/pcp/pcaa086(IF:4.062)
[44] Sun T, Zhang L, Feng J, et al. Characterization of cellular senescence in doxorubicin-induced aging mice. Exp Gerontol. 2022;163:111800. doi:10.1016/j.exger.2022.111800(IF:4.032)
[45] Wang J, Zhang Y, Liu X, Liu H. Is the Fixed Periodic Treatment Effective for the Tumor System without Complete Information?. Cancer Manag Res. 2021;13:8915-8928. Published 2021 Nov 30. doi:10.2147/CMAR.S339787(IF:3.989)
[46] Chen M, Lin H, Gao Y, Wang Z, Li Y, Jin F. Ghrelin attenuates drowning injury via dual effects on damage protection and immune repression. Ann Transl Med. 2021;9(11):920. doi:10.21037/atm-21-795(IF:3.932)
[47] Shi L, Zhang XB, Shi YF, et al. OsCDC48/48E complex is required for plant survival in rice (Oryza sativa L.). Plant Mol Biol. 2019;100(1-2):163-179. doi:10.1007/s11103-019-00851-9(IF:3.928)
[48] Shi J, Wang Y, Chen J, et al. Synthesis and biological evaluation of 1,2,4-oxadiazole core derivatives as potential neuroprotectants against acute ischemic stroke. Neurochem Int. 2021;148:105103. doi:10.1016/j.neuint.2021.105103(IF:3.921)
[49] Zhang KW, Wang D, Cai H, et al. IL‑6 plays a crucial role in epithelial‑mesenchymal transition and pro‑metastasis induced by sorafenib in liver cancer. Oncol Rep. 2021;45(3):1105-1117. doi:10.3892/or.2021.7926(IF:3.906)
[50] Qu F, Song Y, Wu Y, et al. The protective role of Ephrin-B2/EphB4 signaling in osteogenic differentiation under inflammatory environment. Exp Cell Res. 2021;400(2):112505. doi:10.1016/j.yexcr.2021.112505(IF:3.905)
[51] Cheng Q, Fan X, Liu Y, et al. miR-455-5p regulates circadian rhythms by accelerating the degradation of Clock mRNA. IUBMB Life. 2022;74(3):245-258. doi:10.1002/iub.2587(IF:3.885)
[52] He RZ, Zheng JH, Yao HF, et al. ADAMTS12 promotes migration and epithelial-mesenchymal transition and predicts poor prognosis for pancreatic cancer [published online ahead of print, 2022 Apr 25]. Hepatobiliary Pancreat Dis Int. 2022;S1499-3872(22)00117-5. doi:10.1016/j.hbpd.2022.04.005(IF:3.780)
[53] Wang Y, Wang H, Zhang L, et al. Potential mechanisms of tremor tolerance induced in rats by the repeated administration of total alkaloid extracts from the seeds of Peganum harmala Linn. J Ethnopharmacol. 2020;262:113183. doi:10.1016/j.jep.2020.113183(IF:3.690)
[54] Bu S, Li B, Wang Q, et al. Epithelial ovarian cancer stem‑like cells are resistant to the cellular lysis of cytokine‑induced killer cells via HIF1A‑mediated downregulation of ICAM‑1. Int J Oncol. 2019;55(1):179-190. doi:10.3892/ijo.2019.4794(IF:3.571)
[55] Shen Y, Zhou M, Yan J, et al. miR-200b inhibits TNF-α-induced IL-8 secretion and tight junction disruption of intestinal epithelial cells in vitro. Am J Physiol Gastrointest Liver Physiol. 2017;312(2):G123-G132. doi:10.1152/ajpgi.00316.2016(IF:3.468)
[56] Yao C, Chen X, Xu Y, et al. Comparing pretreatment strategies to increase the yield and purity of human urinary extracellular vesicles [published online ahead of print, 2022 Jun 30]. J Chromatogr B Analyt Technol Biomed Life Sci. 2022;1206:123359. doi:10.1016/j.jchromb.2022.123359(IF:3.318)
[57] Cao H, Hua D, Zhang H, et al. Oral immunization of recombinant Saccharomyces cerevisiae expressing fiber-2 of fowl adenovirus serotype 4 induces protective immunity against homologous infection. Vet Microbiol. 2022;271:109490. doi:10.1016/j.vetmic.2022.109490(IF:3.293)
[58] Li Q , Sun J , Mohammadtursun N , Wu J , Dong J , Li L . Curcumin inhibits cigarette smoke-induced inflammation via modulating the PPARγ-NF-κB signaling pathway. Food Funct. 2019;10(12):7983-7994. doi:10.1039/c9fo02159k(IF:3.241)
[59] Yang LY, Liu XF, Yang Y, et al. Biochemical features of the adhesion G protein-coupled receptor CD97 related to its auto-proteolysis and HeLa cell attachment activities. Acta Pharmacol Sin. 2017;38(1):56-68. doi:10.1038/aps.2016.89(IF:3.166)
[60] Fei J, Wu H, Su J, et al. Characterization of MEDLE-1, a protein in early development of Cryptosporidium parvum. Parasit Vectors. 2018;11(1):312. Published 2018 May 23. doi:10.1186/s13071-018-2889-2(IF:3.163)
[61] Yao Y, Luo R, Xiong S, Zhang C, Zhang Y. Protective effects of curcumin against rat intestinal inflammation‑related motility disorders. Mol Med Rep. 2021;23(5):391. doi:10.3892/mmr.2021.12030(IF:2.952)
[62] Li X, An P, Zhao Y, et al. Aqueous Extract and Polysaccharide of Aconiti Lateralis Radix Induce Apoptosis and G0/G1 Phase Cell Cycle Arrest by PI3K/AKT/mTOR Signaling Pathway in Mesangial Cells. Evid Based Complement Alternat Med. 2022;2022:3664696. Published 2022 Apr 22. doi:10.1155/2022/3664696(IF:2.630)
[63] Li D, Han X, Zhao Z, Lu Y, Yang J. Functional analysis of deleterious EPHA2 SNPs in lens epithelial cells. Mol Vis. 2021;27:384-395. Published 2021 Jun 23. (IF:2.367)
[64] You J, Cheng Y, Yang XJ, Chen L. Generation of a homozygous LRP2 knockout human embryonic stem cell line (FDCHDPe010-A-56) by CRISPR/Cas9 system. Stem Cell Res. 2021;53:102342. doi:10.1016/j.scr.2021.102342(IF:2.020)
[65] You J, Xi H, Ma S, Yang XJ, Chen L. Generation of a homozygous LRPAP1 knockout human embryonic stem cell line (FDCHDPe009-B) by CRISPR/Cas9 system. Stem Cell Res. 2021;56:102516. doi:10.1016/j.scr.2021.102516(IF:2.020)
[66] Zhu T, Zhao Y, Wu Y, Qu D. The Staphylococcus epidermidis gdpS regulates biofilm formation independently of its protein-coding function. Microb Pathog. 2017;105:264-271. doi:10.1016/j.micpath.2017.02.045(IF:2.009)
[67] Liu X, Xie F, Lai G, Wang J. Roles of heterogeneous nuclear ribonucleoprotein L in enamel organ development and the differentiation of ameloblasts. Arch Oral Biol. 2020;120:104933. doi:10.1016/j.archoralbio.2020.104933(IF:1.931)
[68] Jiang J, Chen Z, Yang Y, Yan J, Jiang H. Sevoflurane downregulates IGF‑1 via microRNA‑98. Mol Med Rep. 2017;15(4):1863-1868. doi:10.3892/mmr.2017.6219(IF:1.692)
[69] Xia Q, Zhou Y, Wang X, Fu S. Interleukin-1 receptor-associated kinase 3 downregulation in peripheral blood mononuclear cells attenuates immunosuppression in sepsis. Exp Ther Med. 2018;15(2):1586-1593. doi:10.3892/etm.2017.5549(IF:1.261)
[70] Li X, Lei Y, Yu Y, et al. Discovery and characterization of a novel splice variant of the p53 tumor suppressor gene in a human T cell leukemia cellline. Int J Clin Exp Pathol. 2020;13(5):1121-1135. Published 2020 May 1. (IF:0.252)
[71] Feng Y, Jiang C, Yang F, Chen Z, Li Z. Apocynum venetum leaf extract protects against H2O2-induced oxidative stress by increasing autophagy in PC12 cells. Biomed Rep. 2020;13(2):6. doi:10.3892/br.2020.1313(IF:0.000)
[72] Jiaxuan X., Chen C., Meichen D., et al. Ginsenoside Rg3 endows liposomes with prolonged blood circulation and reduced accelerated blood clearance. Journal of Controlled Release(2024). doi:org/10.1016/j.jconrel.2023.10.023
推荐商品
推荐新闻
