产品说明书
FAQ
COA
已发表文献
产品描述
本品是由辣根过氧化酶（HRP）标记的山羊抗小鼠IgG(H+L)，使用抗原偶联的琼脂糖微珠从山羊抗血清内亲和色谱纯化所得。免疫电泳和/或ELISA法检测显示本品特异性结合完整的小鼠IgG分子，也会与其他小鼠免疫球蛋白的轻链结合，可能会与其他物种免疫球蛋白发生交叉反应，但不会识别非免疫球蛋白类的血清蛋白。
本品适合做单标实验。要做多标（Multiple-labeling）实验，建议使用与相近物种血清蛋白或者免疫球蛋白预先经过亲和吸附处理的二抗。
翌圣为您提供Western Blot实验整体解决方案，相关产品选购请参考：WB实验系列产品-选购指南
产品应用
建议稀释浓度：
IHC/ICC：1:250–1:2500；
ELISA/WB（显色法）：1:2500–1:50000
ELISA/WB（化学发光法）：1:5000–1:100000
产品性质
|
抗体浓度（Antibody Concentration） |
100 µL (0.4 mg/mL) |
|
缓冲液（Buffer） |
0.005 M磷酸钠，0.125 M氯化钠，pH 7.6 |
|
稳定剂（Stabilizer） |
7.5 mg/mL BSA（无IgG，蛋白酶），50%甘油 |
|
防腐剂（Preservative） |
None（叠氮化钠会抑制HRP活性） |
|
原料来源（Source of Material） |
Jackson Immunoresearch 115-035-003 |
运输与保存方法
冰袋运输。-20℃分装保存，尽量避免反复冻融。有效期1年。
注意事项
1）本品不能保存于叠氮化钠中，其会抑制辣根过氧化酶（HRP）活性；
2）为了您的安全和健康，请穿实验服并戴一次性手套操作。
3）本产品仅作科研用途！
HB210715
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