泛素蛋白是一个76个氨基酸,高度保守的核和细胞质蛋白。它只存在于真核生物中,通过泛素-蛋白体途径(UPP)中的 酶与底物蛋白共价连接,并在26S蛋白体降解ATP依赖的细胞蛋白中发挥主要作用,泛素化反应可以修饰蛋白质,调节蛋 白质降解。泛素化还影响蛋白质体独立事件,如蛋白质定位、活性和功能。 产品性质 别名(Synonyms)Ubiquitin 来源(Source)E.coli 分子量(MolecularWeight)Approximately10kDa,withanN-terminalMet。 氨基酸序列(AASequence)MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSD YNIQKESTLHLVLRLRGG 外观(PhysicalAppearance)冻干粉lyophilizedpowder 标签(Tag)None 纯度(Purity)>95%bySDS-PAGE 可溶性(Solubility)Aqueoussolutionsupto50mg/ml 复溶(Reconstitution)Werecommendthatthisvialbebrieflycentrifugedpriortoopeningtobringthecontents tothebottom.Reconstituteinsteriledistilledwateroraqueousbuffercontaining0.1% BSAtoaconcentrationof50mg/ml.Stocksolutionsshouldbeapportionedintoworking aliquotsandstoredat≤-20°C.Furtherdilutionsshouldbemadeinappropriatebuffered solutions.
别名(Synonyms) |
Ubiquitin |
来源(Source) |
E.coli |
分子量(Molecular Weight) |
Approximately 10 kDa, with an N-terminal Met。 |
氨基酸序列(AA Sequence) |
MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG |
外观(Physical Appearance) |
冻干粉 lyophilized powder |
标签(Tag) |
None |
纯度(Purity) |
> 95% by SDS-PAGE |
可溶性(Solubility) |
Aqueous solutions up to 50 mg/ml |
复溶(Reconstitution) |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 50 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
冰袋运输。-20℃至-80℃保存,1年有效期。建议第一次使用时分装冻存,避免反复冻融。
Q:该产品如果溶解成5 × Ubiquitin ?
A:泛素每管2.5mg,可以加入500μl无菌水,即为5× Ubiquitin。