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产品信息
|
产品名称 |
产品编号 |
规格 |
|
Recombinant Human Ubiquitin Conjugating Enzyme H5c(UBE2D3/UBCH5C) |
20442ES60 |
100 μg |
产品简介
Recombinant Human UbcH5c/UBE2D3 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application.
产品性质
|
别名(Synonyms) |
UBE2D3, UBCH5C |
|
来源(Source) |
E.coli |
|
分子量 (Molecular Weight) |
Approximately 16 kDa. |
|
氨基酸序列 (AA Sequence) |
MALKRINKELSDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISREWTQKYAM |
|
外观 |
Liquid |
|
标签(Tag) |
None |
|
纯度(Purity) |
> 95% by SDS-PAGE |
|
缓冲液(Buffer) |
1.9 μg/μL in 50 PBS pH 7.0, 150 mM NaCl, 1 mM DTT. See lable for actual concentrration. |
|
使用方法（Use） |
We recommend an initial Recombinant Human UbcH5c/UBE2D3 concentration of 0.1-1 μM. |
储存条件
-25~-15℃保存
注意事项
1. 避免反复冻融。
2. 本产品仅作科研用途。
2. 为了您的安全和健康，请穿实验服并佩戴一次性手套操作。
反应缓冲液配方
|
名称 |
组分 |
浓度 |
|
10 × Reaction Buffer（200 ul） |
Tris PH8.0 |
500 mM |
|
DTT |
6 mM |
|
|
ddH2O |
to 200μL |
|
|
10 x ATP-Mg （200 ul）
|
ATP |
100 mM |
|
MgCl2 |
100 mM |
|
|
ddH2O |
to 200μL |
参考阳性反应体系
|
组分 |
体积 (μL) |
|
ddH2O |
to 20 μL |
|
10 × Reaction Buffer |
2 μL |
|
10 × E1 Enzyme |
2 μL |
|
5 × Ubiquitin |
4 μL |
|
10 × ATP-Mg |
2 μL |
|
20 × E2 Enzyme |
1 μL |
|
10 × E3 Ligase Itch (Positive control) |
2 μL |
|
20 × S5a substrate (Positive control) |
1 μL |
Ver.CN20240329
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